For real time monitoring of the wing state, in this paper, the inverse Finite Element Method (iFEM) is applied, which describes the displacement field of beam according to the Timoshenko theory, to sense the wing frame deformation. In order to maintain the accuracy and stability of frame deformation sensing with iFEM, an optimal placement model of strain sensors based on eigenvalue analysis is constructed. Through the model solution with the Particle Swarm Optimization (PSO) algorithm, two different optimal placement schemes of sensors are obtained. Finally, a simulation is performed on a simple cantilever beam and a static load experiment is conducted on an aluminum alloy wing frame. The results demonstrate that the iFEM is able to accurately sense the deformation of the wing frame, when the two optimal placement schemes of sensors are used.