Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles

doi:10.2217/fvl-2018-0099

CORC > 武汉植物园 > 中国科学院武汉植物园

	Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles
	Zhou, Yuan 3; Wu, Chunchen 3; Chen, Xinwen 3; Hu, Xue 3; Chen, Jizheng 3; Chen, Yingshan 3; Cao, Liang 2,3; Deng, Liu 1,2,3
刊名	FUTURE VIROLOGY
	2018-11-01
卷号	13 期号:11 页码:769-778
关键词	fluorescent protein hepatitis B surface antigen imaging mCherry signal peptide subviral particles
ISSN号	1746-0794
DOI	10.2217/fvl-2018-0099
英文摘要	Aim: To label HBsAg with the mCherry protein without impairing its functionality. Materials & methods: A vector expressing mCherry-HBsAg fusion protein was constructed and transfected into Huh7 cell lines. The expression, secretion and subcellular localization of HBsAg was detected by western blotting, ELISA and immunofluorescence staining, respectively. Then the assembly of subviral particles was evaluated by sucrose density gradient centrifugation, dot blotting and electron microscopic assay. Results: mCherry-HBsAg fusion protein can be expressed and secreted in a similar manner to HBsAg. More importantly, mCherry-HBsAg fusion protein can self-assemble into spherical subviral-like particles. Conclusion: mCherry could be introduced into HBsAg without affecting its biological characters including expression, secretion and assembly.
资助项目	National Nature Science Foundation of China[31770180] ; Youth Innovation Promotion Association CAS[2016303]
WOS研究方向	Virology
语种	英语
出版者	FUTURE MEDICINE LTD
WOS记录号	WOS:000452398000003
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6314]
专题	中国科学院武汉植物园
通讯作者	Zhou, Yuan
作者单位	1.NU, Feinberg Sch Med, Dept Microbiol & Immunol, Chicago, IL USA 2.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yuan,Wu, Chunchen,Chen, Xinwen,et al. Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles[J]. FUTURE VIROLOGY,2018,13(11):769-778.
APA	Zhou, Yuan.,Wu, Chunchen.,Chen, Xinwen.,Hu, Xue.,Chen, Jizheng.,...&Deng, Liu.(2018).Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles.FUTURE VIROLOGY,13(11),769-778.
MLA	Zhou, Yuan,et al."Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles".FUTURE VIROLOGY 13.11(2018):769-778.