Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.

doi:10.3390/molecules24010140

CORC > 武汉植物园 > 中国科学院武汉植物园

	Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.
	Zhang, Yansheng 1,3,4,6; Li, Changfu 1,3,4,6; Zhou, Zilin 2,3,5,6; Li, Xiaohua 2,3,5,6; Zhou, Chen 2,3,5,6
刊名	MOLECULES
	2019
卷号	24 期号:1 页码:14
关键词	Trigonella foenum-graecum L diosgenin biosynthesis glycosyltransferase cytochrome P450 transcriptional factors
ISSN号	1420-3049
DOI	10.3390/molecules24010140
英文摘要	Trigonella foenum-graecum L. (fenugreek) is a valuable resource of producing diosgenin which serves as a substrate for synthesizing more than two hundred kinds of steroidal drugs. Phytochemical analysis indicated that methyl jasmonate (MeJA) efficiently induced diosgenin biosynthesis in fenugreek seedlings. Though early steps up to cholesterol have recently been elucidated in plants, cytochrome P450 (CYP)- and glycosyltransferase (GT)-encoding genes involved in the late steps from cholesterol to diosgenin remain unknown. This study established comparative fenugreek transcriptome datasets from the MeJA-treated seedlings and the corresponding control lines. Differential gene expression analysis identified a number of MeJA-induced CYP and GT candidate genes. Further gene expression pattern analysis across a different MeJA-treating time points, together with a phylogenetic analysis, suggested specific family members of CYPs and GTs that may participate in the late steps during diosgenin biosynthesis. MeJA-induced transcription factors (TFs) that may play regulatory roles in diosgenin biosynthesis were also discussed. This study provided a valuable genetic resource to functionally characterize the genes involved in diosgenin biosynthesis, which will push forward the production of diosgenin in microbial organisms using a promising synthetic biology strategy.
资助项目	National Key R&D Program of China[2018YFC1706200] ; National Natural Science Foundation of China[31670300]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000457150200140
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6169]
专题	中国科学院武汉植物园
通讯作者	Zhang, Yansheng
作者单位	1.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Res Ctr Nat Prod, Shanghai 200444, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 4.Shanghai Univ, Sch Life Sci, Res Ctr Nat Prod, Shanghai Key Lab Bio Energy Crops, Shanghai 200444, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yansheng,Li, Changfu,Zhou, Zilin,et al. Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.[J]. MOLECULES,2019,24(1):14.
APA	Zhang, Yansheng,Li, Changfu,Zhou, Zilin,Li, Xiaohua,&Zhou, Chen.(2019).Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L..MOLECULES,24(1),14.
MLA	Zhang, Yansheng,et al."Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.".MOLECULES 24.1(2019):14.