An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam

doi:10.1016/j.ijbiomac.2018.12.127

	An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam
	Zheng, He 1; Yu, Wei-Li 2; Guo, Xuan 1; Zhao, Yuan-Zhong 1; Cui, Yan 1; Hu, Tao 2; Zhong, Jin-Yi 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-03-15
卷号	125 页码:1016-1023
关键词	Haloalkane dehalogenase Meso-cellular foam Enzyme immobilization
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.12.127
英文摘要	Haloalkane dehalogenase DhaA catalyzes the hydrolysis of halogenated compounds by cleavage of the carbon halogen bond. However, DhaA suffers from poor environmental stability and difficult recovery, which significantly increase the cost of DhaA. Here, an effective enzyme immobilization strategy was developed to overcome the disadvantages of DhaA. DhaA was physically absorbed with amine-functionalized meso-cellular foam (MCF). The MCF-absorbed DhaA (MD) was intermolecularly crosslinked with 8-arm PEG N-hydroxysuccinimide ester and then PEGylated by maleimide-thiol chemistry. DhaA from Rhodococcus rhodochrous was absorbed at a loading capacity of 100 mg/g in MD. The bulk crystallinity and morphology of MCF were largely maintained. The immobilized DhaA (MD-P1-P2) showed a lower Michaelis constant (K-m , 0.588 mM) than DhaA (0.905 mM), along with an extremely low leaching ratio of DhaA (1.1%) from MCF. MD-P1-P2 exhibited a high stability in the extreme environmental conditions, as reflected by the remaining activity of 99.8% in 40% (v/v) DMSO for 5 h, 87.3% in 3 M urea solution for 1 h, 25.9% at pH 3.0, and 51.8% at room temperature for 30 days. Thus, our study was expected to develop an effective immobilized DhaA for practical application. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81703445] ; National Natural Science Foundation of China[81700181]
WOS关键词	ENZYME IMMOBILIZATION ; MESOPOROUS MATERIALS ; MARKEDLY IMPROVES ; SURFACE ; HEXACHLOROCYCLOHEXANE ; STRATEGIES ; CRYSTALLIZATION ; BIODEGRADATION ; STABILIZATION ; STABILITY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000458222000113
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/27849]
专题	中国科学院过程工程研究所
通讯作者	Hu, Tao; Zhong, Jin-Yi
作者单位	1.Res Inst Chem Def, State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zheng, He,Yu, Wei-Li,Guo, Xuan,et al. An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,125:1016-1023.
APA	Zheng, He.,Yu, Wei-Li.,Guo, Xuan.,Zhao, Yuan-Zhong.,Cui, Yan.,...&Zhong, Jin-Yi.(2019).An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,125,1016-1023.
MLA	Zheng, He,et al."An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 125(2019):1016-1023.