Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process

doi:10.1002/aic.16389

	Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process
	Li, Wensong 1; Yang, Liangrong 2; Dong, Tingting 2; Xing, Huifang 2; Wang, Weiyan 1; Yang, Yunquan 1; Liu, Huizhou 2
刊名	AICHE JOURNAL
	2019
卷号	65 期号:1 页码:175-183
关键词	gas-assisted magnetic separation large-scale bio-separation continuous low field separation of proteins
ISSN号	0001-1541
DOI	10.1002/aic.16389
英文摘要	In this study, the gas-assisted low-field magnetic separation (GAMS) was proposed for continuous large-scale magnetic bio-separation process. Magnetic recovery processes in selective separation of binary mixed proteins by magnetic microspheres were selected as a model. Basing on magnetic recovery efficiency of the batch GAMS for this model, the continuous GAMS with an amplifying small pilot scale was further researched. The continuous throughput of GAMS for adsorption, washing, and desorption step reached 12 L/h, 12 L/h, and 18 L/h with an adsorbent recovery above 95%, respectively. Furthermore, magnetic particles via the top magnetic roller could be well-separated from free proteins with good floatability via the bottom outlet of flotation column, and bubbling operation did not cause the significant denaturation of target protein. Compared with classic high gradient magnetic separation, GAMS had the advantages of easy scale-up, easy continuous operation, and low-cost, showing great potential for large-scale magnetic bio-separation process. (c) 2018 American Institute of Chemical Engineers AIChE J, 65: 175-183, 2019
资助项目	National Natural Science Foundation of China[21506180] ; National Natural Science Foundation of China[21676273] ; National Key Natural Science Foundation of China[U1507203] ; National Technology Research and Development Program of China[2015CB251402] ; Science and technology innovation project of CMST[17-163-12-ZD-001-013-01] ; Youth Innovation Promotion Association, CAS[2016043] ; Natural Science Foundation of Hunan Province, China[2016JJ3111]
WOS关键词	IRON-OXIDE NANOPARTICLES ; SUPERPARAMAGNETIC EXTRACTION ; PURIFICATION ; FLOTATION
WOS研究方向	Engineering
语种	英语
出版者	WILEY
WOS记录号	WOS:000454632100017
资助机构	National Natural Science Foundation of China ; National Key Natural Science Foundation of China ; National Technology Research and Development Program of China ; Science and technology innovation project of CMST ; Youth Innovation Promotion Association, CAS ; Natural Science Foundation of Hunan Province, China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/27674]
专题	中国科学院过程工程研究所
通讯作者	Yang, Liangrong; Liu, Huizhou
作者单位	1.Xiangtan Univ, Coll Chem Engn, Xiangtan, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Wensong,Yang, Liangrong,Dong, Tingting,et al. Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process[J]. AICHE JOURNAL,2019,65(1):175-183.
APA	Li, Wensong.,Yang, Liangrong.,Dong, Tingting.,Xing, Huifang.,Wang, Weiyan.,...&Liu, Huizhou.(2019).Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process.AICHE JOURNAL,65(1),175-183.
MLA	Li, Wensong,et al."Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process".AICHE JOURNAL 65.1(2019):175-183.