RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair

	RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair
	Yu, Cheng-han; An, Liwei; Dong, Chao; Li, Junshi; Chen, Jie; Yuan, Jingsong; Huang, Jun; Chan, Kui Ming; Huen, Michael S. Y.
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2018
文献子类	期刊论文
英文摘要	Unrestrained 53BP1 activity at DNA double-strand breaks (DSBs) hampers DNA end resection and upsets DSB repair pathway choice. RNF169 acts as a molecular rheostat to limit 53BP1 deposition at DSBs, but how this fine balance translates to DSB repair control remains undefined. In striking contrast to 53BP1, ChIP analyses of AsiSIinduced DSBs unveiled that RNF169 exhibits robust accumulation at DNA end-proximal regions and preferentially targets resected, RPAbound DSBs. Accordingly, we found that RNF169 promotes CtIPdependent DSB resection and favors homology-mediated DSB repair, and further showed that RNF169 dose-dependently stimulates singlestrand annealing repair, in part, by alleviating the 53BP1-imposed barrier to DSB end resection. Our results highlight the interplay of RNF169 with 53BP1 in fine-tuning choice of DSB repair pathways.
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/13460]
专题	合成所
推荐引用方式 GB/T 7714	Yu, Cheng-han,An, Liwei,Dong, Chao,et al. RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018.
APA	Yu, Cheng-han.,An, Liwei.,Dong, Chao.,Li, Junshi.,Chen, Jie.,...&Huen, Michael S. Y..(2018).RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
MLA	Yu, Cheng-han,et al."RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018).