Manipulation of VEGF-induced angiogenesis by 2-N, 6-O-sulfated chitosan.

	Manipulation of VEGF-induced angiogenesis by 2-N, 6-O-sulfated chitosan.
	Pan, Yuanzhong; Liu, Changsheng; Wang, Jing; Yuan, Yuan; Cao, Lingyan; Zhang, Shuang; Tang, Wei; Sun, Yi; Chen, Rui; Yu, Yuanman
刊名	ACTA BIOMATERIALIA
	2018
文献子类	期刊论文
英文摘要	Emerging evidence suggests that vascular endothelial growth factor (VEGF) is important in the treatment of various ischemic and cardiovascular diseases. However, it often suffers from high cost and easy deactivation with a short half-life. Here, we describe a synthetic 2-N, 6-O-sulfated chitosan (26SCS) with a high affinity to VEGF promoting the binding of the signaling protein to its VEGF receptor 2 (VEGFR2), activating receptor phosphorylation and pro-angiogenic related genes expression, and further stimulating downstream VEGF-dependent endothelial cell viability, migration, tube formation and rat aortic rings outgrowth. Interestingly, the obvious recruitment of mural cells were occurred to stabilize the sprouted microvessels. In addition, the pro-angiogenic potential of 26SCS composited VEGF was confirmed in vivo using the chick embryo chorioallantoic membrane (CAM) assay with an extensive perfusable vascular network. A longer monitoring was administered subcutaneously to mice in a biocompatible gelatin sponge and showed that VEGF with 26SCS had the capability to efficiently enhance neovascularization. These findings highlight that 26SCS, the semi-synthetic natural polymer, may be a promising coagent with VEGF for vascular therapy.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14713]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Pan, Yuanzhong,Liu, Changsheng,Wang, Jing,et al. Manipulation of VEGF-induced angiogenesis by 2-N, 6-O-sulfated chitosan.[J]. ACTA BIOMATERIALIA,2018.
APA	Pan, Yuanzhong.,Liu, Changsheng.,Wang, Jing.,Yuan, Yuan.,Cao, Lingyan.,...&Yu, Yuanman.(2018).Manipulation of VEGF-induced angiogenesis by 2-N, 6-O-sulfated chitosan..ACTA BIOMATERIALIA.
MLA	Pan, Yuanzhong,et al."Manipulation of VEGF-induced angiogenesis by 2-N, 6-O-sulfated chitosan.".ACTA BIOMATERIALIA (2018).