Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles | |
Mingbin Zheng; Xiujun Fan; Lintao Cai; Baozheng Zhang | |
刊名 | Jove-Journal of Visualized Experiments |
2018 | |
文献子类 | 期刊论文 |
英文摘要 | An effective cancer therapeutic method reduces and eliminates tumors with minimal systemic toxicity. Actively targeting nanoparticles offer a promising approach to cancer therapy. The glycosaminoglycan placental chondroitin sulfate A (plCSA) is expressed on a wide range of cancer cells and placental trophoblasts, and malarial protein VAR2CSA can specifically bind to plCSA. A reported placental chondroitin sulfate A binding peptide (plCSA-BP), derived from malarial protein VAR2CSA, can also specifically bind to plCSA on cancer cells and placental trophoblasts. Hence, plCSA-BP-conjugated nanoparticles could be used as a tool for targeted drug delivery to human cancers and placental trophoblasts. In this protocol, we describe a method to synthesize plCSA-BP-conjugated lipid-polymer nanoparticles loaded with doxorubicin (plCSA-DNPs); the method consists of a single sonication step and bioconjugate techniques. In addition, several methods for characterizing plCSA-DNPs, including determining their physicochemical properties and cellular uptake by placental choriocarcinoma (JEG3) cells, are described. |
URL标识 | 查看原文 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.siat.ac.cn:8080/handle/172644/14694] |
专题 | 深圳先进技术研究院_医药所 |
推荐引用方式 GB/T 7714 | Mingbin Zheng,Xiujun Fan,Lintao Cai,et al. Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles[J]. Jove-Journal of Visualized Experiments,2018. |
APA | Mingbin Zheng,Xiujun Fan,Lintao Cai,&Baozheng Zhang.(2018).Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles.Jove-Journal of Visualized Experiments. |
MLA | Mingbin Zheng,et al."Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles".Jove-Journal of Visualized Experiments (2018). |
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