Matrix stiffness regulates the differentiation of tendon-derived stem cells through FAK-ERK1/2 activation

	Matrix stiffness regulates the differentiation of tendon-derived stem cells through FAK-ERK1/2 activation
	Long-Xiang Lin; Yong-Qing Zhuang; Yu-Long Sun*; Chang Liu; Jing-Wan Luo; Ting Liang; Zong-Ping Luo
刊名	EXPERIMENTAL CELL RESEARCH
	2018
文献子类	期刊论文
英文摘要	Tendon derived stem cells (TDSCs) were vital in tendon homeostasis. Nevertheless, the regulation of TDSCs differentiation in tendinopathy is unclear. Matrix stiffness modulated stem cells differentiation, and matrix stiffness of tendinopathic tissues decreased significantly. In order to clarify the role of matrix stiffness in TDSCs differentiaiton, they were cultured on the gelatin hydrogels with the stiffness from 2.34 ± 1.48 kPa to 24.09 ± 14.03 kPa. The effect of matrix stiffness on TDSCs proliferation and differentiation were investigated with CCK8 assay, immunofluorescences, real time PCR and western blot. It was found the proliferation of TDSCs increased and more stress fibers formed with increasing matrix stiffness. The differentiation of TDSCs into tenogenic, chondrogenic, and osteogenic lineages were inhibited on stiff hydrogel evidenced by reduced expression of tenocyte markers THBS4, TNMD, SCX, chondrocyte marker COL2, and osteocyte markers Runx2, Osterix, and ALP. Furthermore, the phosphorylation of FAK and ERK1/2 were enhanced when TDSCs grew on stiff hydrogel. After FAK or ERK1/2 was inhibited, the effect of matrix stiffness on differentiation of TDSCs was inhibited as well. The above results indicated matrix stiffness modulated the proliferation and differentiation of TDSCs, and the regulation effect could correlate to the activation of FAK or ERK1/2.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14436]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Long-Xiang Lin,Yong-Qing Zhuang,Yu-Long Sun*,et al. Matrix stiffness regulates the differentiation of tendon-derived stem cells through FAK-ERK1/2 activation[J]. EXPERIMENTAL CELL RESEARCH,2018.
APA	Long-Xiang Lin.,Yong-Qing Zhuang.,Yu-Long Sun*.,Chang Liu.,Jing-Wan Luo.,...&Zong-Ping Luo.(2018).Matrix stiffness regulates the differentiation of tendon-derived stem cells through FAK-ERK1/2 activation.EXPERIMENTAL CELL RESEARCH.
MLA	Long-Xiang Lin,et al."Matrix stiffness regulates the differentiation of tendon-derived stem cells through FAK-ERK1/2 activation".EXPERIMENTAL CELL RESEARCH (2018).