Preparation and biocompatibility of diphasic magnetic nanocomposite scaffold.

	Preparation and biocompatibility of diphasic magnetic nanocomposite scaffold.
	Li, Long; Duan, Li; Chen, Jielin; Zhu, Feiyan; Lai, Yuxiao; Zhu, Weimin; Jia, ZhaoFeng; You, Wei; Xiong, Jianyi; Wang, Daping
刊名	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2018
文献子类	期刊论文
英文摘要	We describe the study of a new type of diphasic magnetic nanocomposite scaffold (PLGA/Col-I-PLGA/n-HA/Fe2O3) and its preparation using a novel low-temperature deposition manufacturing (LDM) technology. In order to study the biocompatibility of this scaffold, we evaluated and explored its feasibility as a scaffold for tissue engineering. Diphasic magnetic nanocomposite scaffolds (PLGA/Col-I-PLGA/n-HA/Fe2O3) were prepared using LDM technology. The mechanical properties of the scaffold were tested using an electronic testing machine, electron microscopy was utilized to observe the ultrastructure, and a medium (ethanol) immersion method was used to determine the porosity of the scaffold. The scaffold was co-cultured with bone mesenchymal stem cells (BMSCs) and was induced to differentiate. The biocompatibility of the scaffold was then tested. The mechanical test results of the diphasic magneticnanocomposite scaffold demonstrated good mechanical properties. Electron microscopy studies revealed two layers of pore sizes each with a uniform distribution, with the upper cartilage pore size observed to be small while the middle continuous phase was found to be in a good integration. Pore size and porosity test results demonstrated a cartilage layer pore size of 186 mu m, with a porosity measured to be 89.5%. The pore size and porosity of the bone layer were 394 mu m and 86.1%, respectively. These properties met the design requirements of double layer scaffolds. Co-culture of the diphasic magneticnanocomposite scaffold and bone mesenchymal stem cells (BMSCs) exhibited good proliferation of bone mesenchymal stem cells (BMSCs), and the scaffoldwas found to be able to promote differentiation of the differentiation-oriented cells. These results demonstrated a good biocompatibility of the diphasicmagnetic nanocomposite scaffold. The diphasic magnetic nanocomposite scaffold (PLGA/Col-I-PLGA/n-HA/Fe2O3) was found to have suitable mechanical properties as well as cell compatibility. The measured pore size and porosity met the requirements for cell adhesion and cell growth, which matched more closely to that of the physiological structure of normal articular cartilage and subchondral bones. We expect this to represent new technology for improved repair of cartilage and subchondral bone lesions caused by osteoarthritis or trauma.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14421]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Li, Long,Duan, Li,Chen, Jielin,et al. Preparation and biocompatibility of diphasic magnetic nanocomposite scaffold.[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2018.
APA	Li, Long.,Duan, Li.,Chen, Jielin.,Zhu, Feiyan.,Lai, Yuxiao.,...&Liang, Yujie.(2018).Preparation and biocompatibility of diphasic magnetic nanocomposite scaffold..MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS.
MLA	Li, Long,et al."Preparation and biocompatibility of diphasic magnetic nanocomposite scaffold.".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (2018).