Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus)

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus)
	Lv, Biping; Yuan, Lilai; Zha, Jinmiao; Wang, Zijian
刊名	ENVIRONMENTAL TOXICOLOGY
	2017-03-01
卷号	32 期号:3 页码:979-988
关键词	Benzo[a]Pyrene Rare Minnow (Gobiocypris Rarus) P53 Network Genes Gene Expression Histopathology
英文摘要	The p53 pathways play an important role in carcinogenesis. In mammals, p53 and p53 target genes have been extensively studied, but little is known about their functions and regulation in fish. In this study, the cDNA fragments of p53 network genes, including p53, p21, mdm2, gadd45, gadd45, igfbp-3, and bax, were cloned from Chinese rare minnow (Gobiocypris rarus). These genes displayed high amino acid sequence identities with their zebrafish orthologs. The mRNA levels of p53 network genes and pathological changes in the liver were determined after adult rare minnow were exposed to 0.4, 2, and 10 mu g/L of benzo[a]pyrene (BaP) for 28 days. The results showed that p53, p21, mdm2, gadd45, and bax mRNA expressions in the livers from males and females were significantly upregulated compared with those of the controls (p<0.05), but gadd45 and igfbp-3 expression was not significantly changed. Microphotographs revealed enlargement of the cell nuclei and cellular degeneration in males, while atrophy and vacuolization of hepatocytes were observed in females (10 mu g/L). These results suggested that BaP induced liver DNA repair and apoptosis pathways and caused adverse pathological changes in rare minnow. The strongly responsive p53 network genes in the livers suggest that rare minnow is suitable as an experimental fish to screen environmental carcinogens. In addition, the p53 network genes in rare minnow could feasibly be used to identify the mechanism of environmental carcinogenesis. (C) 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 979-988, 2017.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39671]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Lv, Biping,Yuan, Lilai,Zha, Jinmiao,et al. Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus)[J]. ENVIRONMENTAL TOXICOLOGY,2017,32(3):979-988.
APA	Lv, Biping,Yuan, Lilai,Zha, Jinmiao,&Wang, Zijian.(2017).Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus).ENVIRONMENTAL TOXICOLOGY,32(3),979-988.
MLA	Lv, Biping,et al."Benzo[a]pyrene induced p53-mediated cell cycle arrest, DNA repair, and apoptosis pathways in Chinese rare minnow (Gobiocypris rarus)".ENVIRONMENTAL TOXICOLOGY 32.3(2017):979-988.