Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria
	Wang, Xiucheng; Fan, Fenliang; Ma, Xiaotong; Yin, Huaqun; Zhang, Caiwen; Deng, Ye; Feng, Kai; Zhang, Xiaoxia; Zhang, Ruijie; Gao, Jusheng
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2017
卷号	104 期号:0 页码:208-217
关键词	Green Manure Rice Rhizosphere Beneficial Bacteria 16s Rdna Sequencing Quantitative Pcr
英文摘要	Green manure rotation is commonly used to increase soil fertility and improve crop yield. However, the effects of this management practice on the underground microbial ecosystem and the indirect impact on the aboveground crop growth have not been systematically analysed. In this study, we investigated the rice rhizosphere and bulk soil microbial community in a 31-year-old field experimental site treated with different green manures and rice rotations using both 16S rDNA high-throughput sequencing and quantitative PCR approaches. Four treatments have been setup in this experimental site since 1982, including a rice-rice-winter fallow treatment as a control and three green manure rotation treatments: rice-rice-Chinese milk vetch, rice-rice-rape and rice-rice-ryegrass. The qPCR results showed that the bacterial abundances in the rice rhizosphere of the green manure rotation treatments were all significantly higher than in the winter fallow (p < 0.05), but no significant differences were found among those three green manure rotation treatments. Moreover, alpha-diversity analysis revealed that green manure rotations decreased the microbial diversity (Shannon and Simpson indexes) and richness (Chao value) in the rice rhizosphere. Permutational Multivariate Analysis of Variance based on beta-diversity revealed the microbial community was significantly switched in rice rhizosphere after long-term green manure rotation (p < 0.01). Additionally, the soil and plant characteristics contributed almost equally to the rhizosphere bacterial community based on a partial CCA-based variation partitioning analysis. At the genus level, the well-known plant-growth-promoting rhizobacteria Acinetobacter (31%-41%) and Pseudomonas (14%-28%) were the preponderant groups in green manure rotation treatments but accounted for only 4.4% and 2.5% in the winter fallow treatment. Overall, long-term rice-rice-green manure rotation shaped the microbial community in the rice rhizosphere; in particular, some beneficial bacteria, Acinetobacter and Pseudomonas, accumulated in the rhizosphere of green manure treatments. (C) 2016 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39656]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Wang, Xiucheng,Fan, Fenliang,Ma, Xiaotong,et al. Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria[J]. SOIL BIOLOGY & BIOCHEMISTRY,2017,104(0):208-217.
APA	Wang, Xiucheng.,Fan, Fenliang.,Ma, Xiaotong.,Yin, Huaqun.,Zhang, Caiwen.,...&Gao, Jusheng.(2017).Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria.SOIL BIOLOGY & BIOCHEMISTRY,104(0),208-217.
MLA	Wang, Xiucheng,et al."Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria".SOIL BIOLOGY & BIOCHEMISTRY 104.0(2017):208-217.