Phosphate (Pi) and arsenate uptake by two wheat (Triticum aestivum) cultivars and their doubled haploid lines

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	Phosphate (Pi) and arsenate uptake by two wheat (Triticum aestivum) cultivars and their doubled haploid lines
	Smith, F. Andrew; Smith, Sally E.; Tong, Yi-Ping; Geng, Chun-Nu; Zhu, Yong-Guan
刊名	ANNALS OF BOTANY
	2006
卷号	98 期号:3 页码:631-636
关键词	Arsenic Phosphate Transporters Ion Selectivity Plant Membrane Transport
英文摘要	center dot Background and Aims Arsenic accumulation in cereal crops represents an important pathway for human exposure to arsenic from the environment. The objectives of the present work were to find whether the relationship between arsenate and phosphate (Pi) uptake rate differs among genotypes and to select genotypes with a low arsenate uptake rate with the aim of improving food safety and human health. center dot Methods A hydroponic experiment was conducted using two wheat (Triticum aestivum) cultivars (Hanxuan 10 and Lumai 14) and ten doubled haploid (DH) lines derived from them to investigate Pi and arsenate uptake over 48 h. Ten plants were transferred to bottles containing 50 mL of pre-treatment solution containing 0.5 mm CaCl(2) and 5 mm MES set at pH 6.0 with 330 mu m Pi as KH(2)PO(4) and 7.33 mu m arsenate. The solutions were aerated continuously. At 8, 24 and 48 h after uptake, 1 mL of test solution was sampled for determination of Pi and arsenate concentrations. center dot Key Results and Conclusions For each wheat line, Pi and arsenate concentrations in the test solution decreased with uptake time. Exponential (for Pi) or polynomial (for arsenate) regression plots fitted the data closely. For all genotypes, net Pi uptake rates decreased with time (from 0 to 48 h). However, net arsenate uptake rates decreased with time for D5, changed little with time for the male parent, D4 and D6, and increased with time for the others. An inflexion of about 25 mu m Pi was observed for the relationship between arsenate and Pi concentrations in the test solution, indicating that 25 mu m could be the point where the high-affinity uptake system 'switches on', or dominates over low-affinity uptake. In addition, the male parent, D1, D6 and D10 were considered ideal genotypes because they possess Pi transporters that discriminate strongly against arsenate and are expected to accumulate less arsenate in the field.
WOS记录号	WOS:000239898800018
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/23068]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Smith, F. Andrew,Smith, Sally E.,Tong, Yi-Ping,et al. Phosphate (Pi) and arsenate uptake by two wheat (Triticum aestivum) cultivars and their doubled haploid lines[J]. ANNALS OF BOTANY,2006,98(3):631-636.
APA	Smith, F. Andrew,Smith, Sally E.,Tong, Yi-Ping,Geng, Chun-Nu,&Zhu, Yong-Guan.(2006).Phosphate (Pi) and arsenate uptake by two wheat (Triticum aestivum) cultivars and their doubled haploid lines.ANNALS OF BOTANY,98(3),631-636.
MLA	Smith, F. Andrew,et al."Phosphate (Pi) and arsenate uptake by two wheat (Triticum aestivum) cultivars and their doubled haploid lines".ANNALS OF BOTANY 98.3(2006):631-636.