Optimization of key factors affecting biohydrogen production from microcrystalline cellulose by the co-culture of Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2

doi:10.1039/c5ra14192c

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Optimization of key factors affecting biohydrogen production from microcrystalline cellulose by the co-culture of Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2
	Bao, Hongxu; Chen, Chunxiao; Jiang, Lei; Liu, Yichen; Shen, Manli; Liu, Wenzong; Wang, Aijie
刊名	RSC ADVANCES
	2016-01
卷号	6 期号:5 页码:3421-3427
DOI	10.1039/c5ra14192c
英文摘要	The key factors (initial pH value, substrate concentration, incubation time, C/N and L-cysteine concentration) that affect biohydrogen production from microcrystalline cellulose in batch fermentation by the co-culture of isolated strains (Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2) were optimized using an orthogonal experiment. The isolated strain, Clostridium acetobutylicum X-9, had high hydrogen yield from microcrystalline cellulose (MCC), and Ethanoigenens harbinense B-2 could produce hydrogen efficiently from monosaccharides directly from microcrystalline cellulose. The optimal parameters were as follows: initial pH value of 6.0, 12 g L-1 substrate concentration, 40 h incubation time, 0.7 g L-1 L-cysteine concentration and a 4 : 1 ratio of C/N. Under the optimum culture conditions, a maximum hydrogen yield rate of 10.4 mmol g-MCC-1 was obtained. This yield is approximately 2.2-fold greater than that of mono-culture Clostridium acetobutylicum X-9. It suggests that the optimal conditions achieved can be applied to the production of hydrogen from microcrystalline cellulose using the co-culture of isolated strains, Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/36138]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Bao, Hongxu,Chen, Chunxiao,Jiang, Lei,et al. Optimization of key factors affecting biohydrogen production from microcrystalline cellulose by the co-culture of Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2[J]. RSC ADVANCES,2016,6(5):3421-3427.
APA	Bao, Hongxu.,Chen, Chunxiao.,Jiang, Lei.,Liu, Yichen.,Shen, Manli.,...&Wang, Aijie.(2016).Optimization of key factors affecting biohydrogen production from microcrystalline cellulose by the co-culture of Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2.RSC ADVANCES,6(5),3421-3427.
MLA	Bao, Hongxu,et al."Optimization of key factors affecting biohydrogen production from microcrystalline cellulose by the co-culture of Clostridium acetobutylicum X-9 + Ethanoigenens harbinense B-2".RSC ADVANCES 6.5(2016):3421-3427.