Tetrabromoethylcyclohexane affects gonadal differentiation and development in the frog Pelophylax nigromaculatus

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Tetrabromoethylcyclohexane affects gonadal differentiation and development in the frog Pelophylax nigromaculatus
	Chen, Juan; Qin, Zhan-Fen; Li, Xing-Hong; Cai, Man; Li, Yuan-Yuan; Meng, Tan; Liu, Peng-Yan
刊名	AQUATIC TOXICOLOGY
	2017-10-01
卷号	192 期号:0 页码:40-47
关键词	Tetrabromoethylcyclohexane Pelophylax Nigromaculatus Masculinization Gonadal Differentiation Androgenic Chemical
英文摘要	Tetrabromoethylcyclohexane (TBECH), an additive brominated flame retardant, has been shown to have an androgenic activity in vitro. In the present study, we aimed to investigate the effects of TBECH on gonadal differentiation and development in the frog Pelophylax nigromaculatus, an amphibian species sensitive to androgenic chemicals, and to assess the androgenic activity of TBECH in vivo. P. nigromaculatus tadpoles were exposed to TBECH (1, 10, 100 nM) from Gosner stage 24 to complete metamorphosis, and to 5 alpha-dihy-drotestosterone (DHT) as a positive control. We found that 1 nM DHT resulted in 100% males, while the sex ratio in the solvent control group was close to 1:1. In all the TBECH treatment groups, sexually ambiguous gonads based on gross morphology and intersexualities with testicular and ovarian histological structures were found, but no abnormality occurred in the solvent control. In the 1, 10, 100 nM TBECH treatment groups, the female percentages were 52%, 31%, 17%, with 36%, 56%, 66% for males and 12%, 13%, 17% for abnormal sexes, respectively. X-2-test revealed significant differences in sex ratios between the three TBECH groups and the solvent control group, and the sex ratios in the two higher concentration groups were male-biased. These observations show that TBECH has a masculinizing effect on gonadal differentiation and development in P. nigromaculatus, suggesting an androgenic activity of TBECH in vivo. To our knowledge, this is the first study demonstrating that TBECH could induce gonadal masculinization in an animal, which raises new concerns for reproductive risk of TBECH exposure.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39430]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Chen, Juan,Qin, Zhan-Fen,Li, Xing-Hong,et al. Tetrabromoethylcyclohexane affects gonadal differentiation and development in the frog Pelophylax nigromaculatus[J]. AQUATIC TOXICOLOGY,2017,192(0):40-47.
APA	Chen, Juan.,Qin, Zhan-Fen.,Li, Xing-Hong.,Cai, Man.,Li, Yuan-Yuan.,...&Liu, Peng-Yan.(2017).Tetrabromoethylcyclohexane affects gonadal differentiation and development in the frog Pelophylax nigromaculatus.AQUATIC TOXICOLOGY,192(0),40-47.
MLA	Chen, Juan,et al."Tetrabromoethylcyclohexane affects gonadal differentiation and development in the frog Pelophylax nigromaculatus".AQUATIC TOXICOLOGY 192.0(2017):40-47.