Metabolically Generated Stable Isotope-Labeled Deoxynucleoside Code for Tracing DNA N-6-Methyladenine in Human Cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Metabolically Generated Stable Isotope-Labeled Deoxynucleoside Code for Tracing DNA N-6-Methyladenine in Human Cells
	Liu, Baodong; Liu, Xiaoling; Lai, Weiyi; Wang, Hailin
刊名	ANALYTICAL CHEMISTRY
	2017-12-01
卷号	89 期号:11 页码:6203-6210
英文摘要	DNA N-6-methyl-2'-deoxyadenosine (6mdA) is an epigenetic modification in both eukaryotes and bacteria. Here we exploited stable isotope -labeled deoxynucleoside [N-15(5)]-2'-deoxyadenosine ([N-15(5)]-dA) as an initiation tracer and for the first time developed a metabolically differential tracing code for monitoring DNA 6mdA in human cells. We demonstrate that the initiation tracer [N-15(5)]-dA undergoes a specific and efficient adenine deamination reaction leading to the loss the exocyclic amine N-15, and further utilizes the purine salvage pathway to generate mainly both [N-15(4)]-dA and [N-15(4)]-2'-deoxyguanosine ([N-15(4)]-dG) in mammalian genomes. However, [N-15(5)]-dA is largely retained in the genomes of mycoplasmas, which are often found in cultured cells and experimental animals. Consequently, the methylation of dA generates 6mdA with a consistent coding pattern, with a predominance of [N-15(4)]-6mdA. Therefore, mammalian DNA 6mdA can be potentially discriminated from that generated by infecting mycoplasmas. Collectively, we show a promising approach for identification of authentic DNA 6mdA in human cells and determine if the human cells are contaminated with mycoplasmas.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39361]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Baodong,Liu, Xiaoling,Lai, Weiyi,et al. Metabolically Generated Stable Isotope-Labeled Deoxynucleoside Code for Tracing DNA N-6-Methyladenine in Human Cells[J]. ANALYTICAL CHEMISTRY,2017,89(11):6203-6210.
APA	Liu, Baodong,Liu, Xiaoling,Lai, Weiyi,&Wang, Hailin.(2017).Metabolically Generated Stable Isotope-Labeled Deoxynucleoside Code for Tracing DNA N-6-Methyladenine in Human Cells.ANALYTICAL CHEMISTRY,89(11),6203-6210.
MLA	Liu, Baodong,et al."Metabolically Generated Stable Isotope-Labeled Deoxynucleoside Code for Tracing DNA N-6-Methyladenine in Human Cells".ANALYTICAL CHEMISTRY 89.11(2017):6203-6210.