Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes
	Zhao, Yanhong; Xu, Kai; Chen, Xia; Yang, Hui; Xu, Yiwen; He, Yuanlin; Wang, Chenfei; Huang, Hua; Liu, Baodong; Liu, Wenqiang
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2017-04-01
卷号	292 期号:5 页码:1798-1807
关键词	Dna Methylation Embryo Histone Methylation Meiosis Oocyte
英文摘要	Sall4 (Splat-like 4) plays important roles in maintaining pluripotency of embryonic stem cells and in various developmental processes. Here, we find that Sall4 is highly expressed in oocytes and early embryos. To investigate the roles of SALL4 in oogenesis, we generated Sall4 maternal specific knock-out mice by using CRISPR/Cas9 system, and we find that the maternal deletion of Sall4 causes developmental arrest of oocytes at germinal vesicle stage with non-surrounded nucleus, and the subsequent meiosis resumption is prohibited. We further discover that the loss of maternal Sall4 causes failure in establishment of DNA methylation in oocytes. Furthermore, we find that Sall4 modulates H3K4me3 and H3K27me3 modifications by regulating the expression of key histone demethylases coding genes Kdm5b, Kdm6a, and Kdm6b in oocytes. Moreover, we demonstrate that the aberrant H3K4me3 and H3K27me3 cause mis-expression of genes that are critical for oocytes maturation and meiosis resumption. Taken together, our study explores a pivotal role of Sall4 in regulating epigenetic maturation of mouse oocytes.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39355]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhao, Yanhong,Xu, Kai,Chen, Xia,et al. Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017,292(5):1798-1807.
APA	Zhao, Yanhong.,Xu, Kai.,Chen, Xia.,Yang, Hui.,Xu, Yiwen.,...&Gao, Shaorong.(2017).Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes.JOURNAL OF BIOLOGICAL CHEMISTRY,292(5),1798-1807.
MLA	Zhao, Yanhong,et al."Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes".JOURNAL OF BIOLOGICAL CHEMISTRY 292.5(2017):1798-1807.