Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers

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	Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers
	Wang ZH(王战会); Viana AS; Jin G(靳刚); Abrantes LM
刊名	Bioelectrochemistry
	2006-10
通讯作者邮箱	luisa.abrantes@fc.ul.pt
卷号	69 期号:2 页码:180-186
关键词	Mixed self-assembled monolayers Immunosensor Human immunoglobulin G Tween 20 Electrochemistry Ellipsometry
ISSN号	1567-5394
通讯作者	Abrantes, LM (reprint author), Univ Lisbon, Fac Ciencias, Lab SPM, Campo Grande, P-1749016 Lisbon, Portugal.
合作状况	国际
中文摘要	An immunosensor interface based on mixed hydrophobic self-assembled monolayers (SAMs) of methyl and carboxylic acid terminated thiols with covalently attached human Immunoglobulin G (hIgG), is investigated. The densely packed and organised SAMs were characterised by contact angle measurements and cyclic voltammetry. The effect of the non-ionic surfactant, Tween 20, in preventing nonspecific adsorption is addressed by ellipsometry during physical and covalent hIgG immobilization on pure and mixed SAMs, respectively. It is clearly demonstrated that nonspecific adsorption due to hydrophobic interactions of hIgG on methyl ended groups is totally inhibited, whereas electrostatic/hydrogen bonding interactions with the exposed carboxylic groups prevail in the presence of surfactant. Results of ellipsometry and Atomic Force Microscopy, reveal that the surface concentration of covalently immobilized hIgG is determined by the ratio of COOH/CH3-terminated thiols in SAM forming solution. Moreover, the ellipsometric data demonstrates that the ratio of bound anti-hIgG/hIgG depends on the density of hIgG on the surface and that the highest ratio is close to three. We also report the selectivity and high sensitivity achieved by chronoamperometry in the detection of adsorbed hIgG and the reaction with its antibody.
学科主题	力学
类目[WOS]	Biochemistry & Molecular Biology ; Biology ; Biophysics ; Electrochemistry
研究领域[WOS]	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry
关键词[WOS]	SURFACE-PLASMON RESONANCE ; SCANNING FORCE MICROSCOPY ; PROTEIN ADSORPTION ; ALKANETHIOLATE MONOLAYERS ; POLY(ETHYLENE GLYCOL) ; REDUCTIVE DESORPTION ; GOLD ELECTRODES ; MODEL SURFACES ; IMMOBILIZATION ; ANTIBODY
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.bioelechem.2006.02.001
语种	英语
WOS记录号	WOS:000242005800007
公开日期	2007-06-15 ; 2007-12-05 ; 2009-06-23
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/17676]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Wang ZH,Viana AS,Jin G,et al. Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers[J]. Bioelectrochemistry,2006,69(2):180-186.
APA	Wang ZH,Viana AS,Jin G,&Abrantes LM.(2006).Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers.Bioelectrochemistry,69(2),180-186.
MLA	Wang ZH,et al."Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers".Bioelectrochemistry 69.2(2006):180-186.