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	A New RP-RPLC Approach for Large-scale Phosphorylation Analysis of Human Liver.
	Song CX(宋春侠) ; Han GH(韩广辉) ; Jiang XN(江新宁) ; Yu ZY(于志远) ; Wang FJ(王方军) ; Chen R(陈锐) ; Ye ML(叶明亮) ; Zou HF(邹汉法)
	2010-08-29
会议名称	17th international symposium on electro- and liquid phase-separation techniques
会议日期	2010-8-29
会议地点	美国
页码	68/2
通讯作者	邹汉法
中文摘要	as the best-known post translational modifications (ptms), protein phosphorylation has reached the stage where its importance in almost every life event is recognized. however, our understanding of protein phosphorylation is still in its infancy. the greatest challenge of phosphorylation analysis is due to its highly dynamic nature and low abundance. therefore it is crucial to develop new analytical methods for phosphorylation analysis to separate the phosphorylated peptides from the complex samples. herein a new reversed-phase-reversed-phase liquid chromatography (rp-rplc) approach was developed for multidimensional separation of phosphopeptides. combination of this multidimensional system, immobilized metal affinity chromatography, high-accuracy mass spectrometry instrumentation, and a new data processing platform called armone, a total of 9,995 phosphorylation sites and 3149 phosphoproteins from 40mg tryptic digestion of human liver lysate were identified with an fdr 1.0%. to our knowledge, this is the largest data set for human liver phosphoproteome. this ph-based rp-rplc technology allows a higher coverage of phosphoproteome and holds a promising in large-scale analysis of protein phosphorylation. keywords: phosphorylation analysis / multidimensional chromatography / phosphopeptide / human liver / ph-reversed phase / phosphoproteomics. references: 1. cohen, p. nat cell biol.2002, 4, e127-e130. 2. mclachlin, d. t.; chait, b. t. curr opin chem biol.2001, 5, 591-602. 3. paradela, a; albar, j. p,. j. proteome res. 2008, 7, 1809-1818. 4. gilar, m; olicoca, p; daly, a. e; gebler, j. c. anal. chem, 2005, 77, 6426-6434. 5. villen, j; beausoleil, s.a; gerber, s.a; gygi, s.p. proc. natl. acad. sci. usa, 2007, 104, 1488-1493.
会议主办者	oklahoma state university
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/114156]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Song CX,Han GH,Jiang XN,et al. A New RP-RPLC Approach for Large-scale Phosphorylation Analysis of Human Liver.[C]. 见:17th international symposium on electro- and liquid phase-separation techniques. 美国. 2010-8-29.

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