| High Efficient Proteome Analysis with Gel Free Protein Prefractionation and Peptide Separation | |
| Tao DY(陶定银) ; Zhang LH(张丽华) ; Liang Z(梁振) ; Zhang YK(张玉奎) | |
| 2009-09-26 | |
| 会议名称 | hupo 8th annual world congress |
| 会议日期 | 2009-9-26 |
| 会议地点 | 加拿大 |
| 页码 | 129/1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | analysis of whole proteome is an extraordinary challenge because of the complexity and wide dynamic range of proteins,therefore protein prefractionation is pre-requisite to achieve high efficient proteome analysis.in this study gel-free protein fractionation by preparative microscale solution isoelectric focusing(pmsief).off-line protein digestion,peptide separation by 2d-scx-rplc with serially coupled long microcolumn, and protein identification by esi-ms/ms were combined,by which 1852 proteins were identified from rat brain. |
| 会议主办者 | york university |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| ISSN号 | 1542－6416 |
| 内容类型 | 会议论文 |
| 源URL | [http://159.226.238.44/handle/321008/113602]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Tao DY,Zhang LH,Liang Z,et al. High Efficient Proteome Analysis with Gel Free Protein Prefractionation and Peptide Separation[C]. 见:hupo 8th annual world congress. 加拿大. 2009-9-26. |
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