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	Protein fractioned by Microscale Solution Isoelectric Focusing and Identification by μHPLC-MS/MS
	Tao DY(陶定银) ; Duan JC(段继诚) ; Liang Z(梁振) ; Zhang WB(张维冰) ; Zhang LH(张丽华) ; Zhang YK(张玉奎)
	2008-08-16
会议名称	7th Annual World Congress HUPO2008
会议日期	2008-8-16
会议地点	Netherlands
页码	无/2
通讯作者	张丽华
中文摘要	Comprehensive analysis of whole proteomes is an extraordinary challenge because of the complexity and wide dynamic range of proteins. The success of proteome analysis is highly dependent on the quality of sample prefractionation prior to the identification by MS/MS. In our recent work, preparative microscale solution isoelectric focusing (PMSIEF) was used to achieve narrow-band fractionation of proteins from Escherichia coli (E. coli). Proteins in various pH related fractions were further separated using coupled long reversed phase high-performance liquid chromatography (RP-HPLC) columns and identified by MS/MS.
会议主办者	HUPO Council
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113382]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Tao DY,Duan JC,Liang Z,et al. Protein fractioned by Microscale Solution Isoelectric Focusing and Identification by μHPLC-MS/MS[C]. 见:7th Annual World Congress HUPO2008. Netherlands. 2008-8-16.

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