An Integrated M-IPG-CIEF-microenzymatic reactor-CZE platform for protein analysis
Wang TT(王婷婷) ; Liang Z(梁振) ; Zhang LH(张丽华) ; Zhang YK(张玉奎)
2008-08-16
会议名称7th annual world congress hupo2008
会议日期2008-8-16
会议地点netherlands
页码无/2
通讯作者张丽华
中文摘要although 2d-page has played extremely important role in proteome study, it still suffers from disadvantages for the separation of extremely acidic and basic proteins, and solid phase based operation. therefore, in recent years, much effort has been made on 2d-capillary electrophoresis (ce) to provide complementary platforms. recently, dovichi et al integrated the intact proteins separation first by cse, on-line digestion by pepsin immobilized microreactor, the separation of resulting peptides by mekc and the protein identified by ms/ms, and showed the promising of the combination of top-down and bottom-up strategies (1). in our recent work, a novel ce based integrated platform for protein study was established.
会议主办者hupo council
学科主题物理化学
语种中文
内容类型会议论文
源URL[http://159.226.238.44/handle/321008/113380]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式
GB/T 7714
Wang TT,Liang Z,Zhang LH,et al. An Integrated M-IPG-CIEF-microenzymatic reactor-CZE platform for protein analysis[C]. 见:7th annual world congress hupo2008. netherlands. 2008-8-16.
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