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	Involvement of N-mediated defense in oligochitosan inducing resistance to tobacco mosaic virus
	Lu H(卢航) ; Zhao XM(赵小明) ; Bai XF(白雪芳) ; Du YG(杜昱光)
	2008-10-12
会议名称	the 13th international biotechnology symposium
会议日期	2008-10-12
会议地点	中国
页码	s581/2
通讯作者	杜昱光
中文摘要	oligochitosanwas applied by spraying it on tobacco leaves for inhibition of tobacco mosaic virus (tmv). the maximum inhibition of tmv by oligochitosan was observed when inoculation occurred at 24 h after spraying 50 g/ml oligochitosan (zhao et al., 2006). but the mechanism is unknown. the tobacco n gene, a member of the toll-interleukin 1 homology region/nucleotide binding site/leucine-rich repeat (tir-nbs-lrr) class of resistance (r) gene, confers resistance to tobacco mosaic virus (tmv) (liu et al., 2004). in this report, we investigated the roles of some genes involved in n signaling, after oligchitosan treating tobacco plants. we used rt-pcr to identify changes of these known defense genes. it was found oligochitosan induced sgt1 and eds1 gene expression, any of which is required for n function. a mitogen-activated protein kinase (mapk) oipk, or an mapk kinase (mapkk) ntmek2, was up-regulated by oligochitosan. onemember of wrky transcription factors, tizz, which is concerned with n-mediated lesion forming was also induced by oligochitosan. silencing of the oipk gene attenuated oligochitosan-inducing resistance to tmv (feng et al., 2006). we also found silencing oipk altered oligochitosan-triggering genes expression, and further silenced tizz. our results suggest that oligochitosan inducing tobacco defense is involved with n-mediated resistance.
会议主办者	中国科学院 中国工程院 中华人民共和国教育部 中华人民共和国科学技术部 中华人民共和国国家发展改革委员会
学科主题	物理化学
语种	中文
WOS记录号	WOS:000208979402326
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113244]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Lu H,Zhao XM,Bai XF,et al. Involvement of N-mediated defense in oligochitosan inducing resistance to tobacco mosaic virus[C]. 见:the 13th international biotechnology symposium. 中国. 2008-10-12.

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