| Purification of chitooligosaccharides by using purification factory | |
| Guo ZM(郭志谋) ; Xiao YS(肖远胜) ; Liu YF(刘艳芳) ; Liang T(梁图) ; Liang XM(梁鑫淼) | |
| 2008-10-12 | |
| 会议名称 | 13th international biotechnology symposium & exhibition |
| 会议日期 | 2008-10-12 |
| 会议地点 | 中国 |
| 其他题名 | 纯化分离工厂制备壳寡糖 |
| 页码 | s83/2 |
| 通讯作者 | 梁鑫淼 |
| 中文摘要 | oligosaccharides are being increasingly identified with the biological activities. chitooligosaccharides (cos), a type of specifically alkali oligosaccharides prepared by chemical and enzymatic hydrolysis of chitosan, was found to have antimicrobial, antibacterial, antitumour and antiangiogenesis properties [1]. but the biological screening is usually performed by using mixture of cos with different degree of polymerization [2], which is an unfavorable way to discover exactly active compounds. separation and purification of cos is quite difficult due to the highly polarity. a new technique for purification of cos with high efficiency was developed by using purification factory, which is a 4-channel parallel preparative high performance liquid chromatography (hplc) system. hydrophilic interaction chromatography (hilic), an effective hplc mode for separation of very polar compounds including oligosaccharides [3], was employed to obtain enough retention and resolution of cos under optimized conditions. four fractions were collected by using mass spectrometry (ms) as collection trigger. the four fractions are four kinds of cos with different degree of polymerization, which is 2, 3, 4, 5, respectively. this new purification technique, together with the purified products, will be very helpful to the research on oligosaccharides. references: [1] chung, y. c., kuo, c. l. and chen, c. c. 2005. preparation and important functional properties of water-soluble chitosan produced through maillard reaction. bioresource technology, 13, 1473-1482. [2] dou, j. l., tan, c. y., du, y. g., bai, x. f., wang, k. y. and ma, x. j. 2007. effects of chitooligosaccharides on rabbit neutrophils in vitro. carbohydrate polymers, 69, 209-213. [3] hemstrom, p. and irgum, k. 2006. hydrophilic interaction chromatography. journal of separation science, 29, 1784-1821. |
| 会议主办者 | 中国科学院、中国工程院、教育部、科技部和国家发改委 |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| WOS记录号 | WOS:000208979400193 |
| 内容类型 | 会议论文 |
| 源URL | [http://159.226.238.44/handle/321008/113154]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Guo ZM,Xiao YS,Liu YF,et al. Purification of chitooligosaccharides by using purification factory[C]. 见:13th international biotechnology symposium & exhibition. 中国. 2008-10-12. |
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