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	Experimental studies of the role of different cell types in morphogenesis in marine sponge Hymeniacidon perleve
	Sun LM(孙黎明) ; Zhang W(张卫) ; Yu XJ(虞星炬) ; Jin MF(金美芳)
	2008-10-12
会议名称	13th international biotechnology symposium and exhibition
会议日期	2008-10-12
会议地点	中国
页码	s110/2
通讯作者	孙黎明
中文摘要	sponges (demonspongia, porifera), the lowest multi-cellular invertebrate animals-sponges are an important model for the research of cell–cell interaction (imsiecke et al., 1995; koziol et al., 1998). archaeocyte is generally considered as the toti/multipotent “stem cells” in sponges. they have the capacity to proliferate and to differentiate into other cell types. to investigate the interaction between archaeocyte with other predominant cell types in marine sponge, such as pinacocyte and collencyte, we separated the archaeocytes, pinacocytes and collencytes from the marine sponge hymeniacidon perleve by a method composed of differential centrifugation, selective agglomeration, differential adhesion and ficoll-vrografin density gradient centrifugation (sun et al., 2007). the pinacocytes and collencytes (in a ratio of 1:1, denoted together as pcs) mixed with archaeocytes in ratio of 1:2, 1:1 and 2:1, respectivelywere cultured in seawater. the results showed that pcs could aggregate and adhere to the substratum, but they could not develop into small sponge. archaeocytes could form suspended aggregates, not functional sponges. when pcs and archaeocytes were mixed together, especially in a ratio of 2:1, almost all of the sponge cells aggregated and adhered quickly. moreover, a series of self-organization and differentiation phenomena could be observed, such as secretion of collagen fibre and silicon spicules, construction of canal systems, outward movement of archaeocytes and final formation of functional sponges. it is suggested that the pcs may play an important role in regulation the biological behaviour, especially differentiation of the archaeocytes and even the morphogenesis of sponge larva.
会议主办者	中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委
学科主题	物理化学
语种	中文
WOS记录号	WOS:000208979400255
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113106]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Sun LM,Zhang W,Yu XJ,et al. Experimental studies of the role of different cell types in morphogenesis in marine sponge Hymeniacidon perleve[C]. 见:13th international biotechnology symposium and exhibition. 中国. 2008-10-12.

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