| Comparison of spiculogenesis in in vitro ADCP-primmorph and explawith 3-TMOSPU supplementation.nts culture of marine sponge Hymeniacidon perleve; 3-TMOSPU添加对繁茂膜海绵外植体和细胞培养过程中骨针发生的影响 | |
| Zhang W(张卫) ; Cao XP(曹旭鹏) | |
| 2007-09-16 | |
| 会议名称 | 13th european congress on biotechnology |
| 会议日期 | 2007-9-16 |
| 会议地点 | 西班牙 |
| 其他题名 | 3-tmospu添加对繁茂膜海绵外植体和细胞培养过程中骨针发生的影响 |
| 页码 | 75/1 |
| 通讯作者 | 张卫 |
| 中文摘要 | tocharacterizetheformationofsilicaspicules,thedynamicsofspiculogenesisofanintertidalmarinespongehymeniacidonperlevis(montagu1818)(porifera:demospongiae)wereinvestigatedbymeasuringthegeneexpressionofsilicatein(theenzymeresponsibleforspiculesilicification)andthedimensionalchangesofspiculesduringthedevelopmentalprocessofindividualspongesandincellculturesofprimmorphsofarchaeocyte-dominantcellpopulations.thedifferentdevelopmentalstagesofspiculesweredocumentedbytime-lapsemicroscopyandobservedbytransmissionelectronmicroscopyduringa1-monthcultureperiod.duringitsannuallifecycle,h.perlevishasfourdifferentdevelopmentalstages: dormancy,resuscitation,bloom,anddecline.field-grownindividualspongesamplesatdifferentstageswerecollectedover7months(marchtoseptember2005).thedimensionsofthesilicaspiculesfromthesesamplesweremicroscop¬icallymeasuredandstatisticallyanalyzed.thisanalysisandthematerialpropertiesofthespiculesallowedthemtobeclassifiedintofourgroupsrepresentingthedifferentdevelopmentalstagesofspiculogenesis.silicateinexpres¬sioninthebloomstagewasmorethan100timeshigherthanthatintheotherstagesandwascorrelatedwiththespiculedevelopmentalstage.thetrendofspiculeformationinfield-grownspongeswasconsistentwiththetrendincellculture.anewparameter,thematurationdegree(md)ofspicules(definedastheratioofactualtotheoreticalsilicadepositionofmaturespicules),wasintroducedtoquantifyspiculedevelopment.silicaspiculogenesisduringh.perlevisdevelopmentwasdelineatedbycomparingmdandsilicateinexpression. keywordssilicaspicule.silicatein.spiculogenesis.archaeocyte-dominantcellpopulations.primmorphculture.sponge,hymeniacidonperlevis(porifera) |
| 会议主办者 | 西班牙 |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| 内容类型 | 会议论文 |
| 源URL | [http://159.226.238.44/handle/321008/112606]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang W,Cao XP. Comparison of spiculogenesis in in vitro ADCP-primmorph and explawith 3-TMOSPU supplementation.nts culture of marine sponge Hymeniacidon perleve, 3-TMOSPU添加对繁茂膜海绵外植体和细胞培养过程中骨针发生的影响[C]. 见:13th european congress on biotechnology. 西班牙. 2007-9-16. |
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