Oligochitosan-responsive genes in Nicotiana tabacum var.Samsun NN; 枯斑三生烟草中的壳寡糖响应基因 | |
Zhang FY(张付云) ; Feng B(冯斌) ; Bai XF(白雪芳) ; Du YG(杜昱光) ; Zhang YK(张玉奎) | |
2005-09-17 | |
会议名称 | 13th international congress on genes, gene families and isozymes 2005''forum on fishery science and technology |
会议日期 | 2005-9-17 |
会议地点 | 中国 |
其他题名 | 枯斑三生烟草中的壳寡糖响应基因 |
页码 | 107/1 |
通讯作者 | 杜昱光 |
中文摘要 | to provide an insight into the molecular basis of plant resistance induced by oligochitosan, mrna differential display was used to analyze the difference of gene expression between tobacco treated with oligochitosan and untreated nicotiana tabacum (var. samsun nn) and three enhanced-expression gene fragments were obtaind. these fragments were re-amplifed and purified before they were subclond into pmd18-t vector. the result of analysis of reverse northern blot indicated that the expression of the three genes was enhanced by oligochitosan induction. sequence and homology analysis show that clone 32 share 90% identity with capsicum annuum mrna for plastid transketolase 1,clone 68 has 59% homology to arabidopsis thaliana homeodomain protein protodermal factor 2 (pdf2), and clone 70 showes 84% identity to arabidopsis thaliana vacuolar atpase-related (at4g02320) mrna in nucleotide sequence. |
学科主题 | 物理化学 |
语种 | 中文 |
内容类型 | 会议论文 |
源URL | [http://159.226.238.44/handle/321008/111842] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
推荐引用方式 GB/T 7714 | Zhang FY,Feng B,Bai XF,et al. Oligochitosan-responsive genes in Nicotiana tabacum var.Samsun NN, 枯斑三生烟草中的壳寡糖响应基因[C]. 见:13th international congress on genes, gene families and isozymes 2005''forum on fishery science and technology. 中国. 2005-9-17. |
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