High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans

	High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans
	Dou, Quan-Wen 1; Chen, Zhi-Guo 1; Liu, Yong-An 1; Tsujimoto, Hisashi 2
刊名	breeding science
	2009-12-01
卷号	59 期号:5 页码:651-656
关键词	perennial grass forage Elymus nutans karyotype variation FISH GISH
ISSN号	1344-7610
中文摘要	elymus nutans l. (sthy, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the qinghai-tibet plateau in china due to its high tolerance to environmental stresses, such as cold and drought. karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (fish) and genomic in situ hybridization (gish). gish discriminated st, h and y genomes, indicating that e. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. however, fish using an agg satellite and afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. in addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. the nature of the polymorphisms seen in the chromosomes of the domesticated population of e. nutans is discussed.
英文摘要	elymus nutans l. (sthy, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the qinghai-tibet plateau in china due to its high tolerance to environmental stresses, such as cold and drought. karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (fish) and genomic in situ hybridization (gish). gish discriminated st, h and y genomes, indicating that e. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. however, fish using an agg satellite and afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. in addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. the nature of the polymorphisms seen in the chromosomes of the domesticated population of e. nutans is discussed.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	agronomy ; plant sciences
研究领域[WOS]	agriculture ; plant sciences
关键词[WOS]	in-situ hybridization ; repetitive sequences ; afa-family ; triticeae ; poaceae ; identification ; genome ; barley ; leymus ; wheat
收录类别	SCI ; ISTP
语种	英语
WOS记录号	WOS:000274757200026
公开日期	2011-12-13
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn//handle/363003/1788]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Tottori Univ, Fac Agr, Tottori 6808550, Japan
推荐引用方式 GB/T 7714	Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,et al. High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans[J]. breeding science,2009,59(5):651-656.
APA	Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,&Tsujimoto, Hisashi.(2009).High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans.breeding science,59(5),651-656.
MLA	Dou, Quan-Wen,et al."High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans".breeding science 59.5(2009):651-656.