2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification

	2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification
	You, Jinmao 1,2; Fu, Yanyan 1; Sun, Zhiwei 1,3; Suo, Yourui 1
刊名	analytical and bioanalytical chemistry
	2010-04-01
卷号	396 期号:7 页码:2657-2666
关键词	2-(5-Benzoacridine)ethyl-p-toluenesulfonate Bile acids Column liquid chromatography Fluorescence detection Mass spectrometry
ISSN号	1618-2642
中文摘要	2-(5-benzoacridine)ethyl-p-toluenesulfonate (baets), a dual-sensitive probe, was reacted with bile acids in the presence of k(2)co(3) catalyst in dimethyl sulfoxide (dmso) solvent to give baets-bile acid derivatives. derivatives exhibited intense fluorescence (fl) with an excitation maximum lambda(ex) 270 nm and an emission maximum lambda(em) 510 nm. ms analysis using apci-ms indicated that derivatives had excellent apci-ms ionizability with percentage ionization delta values changing from 0 to 88.83% in aqueous acetonitrile and from 0 to 89.15% in aqueous methanol. the collision induced dissociation spectra of m/z [m + h](+) contained specific fragment ions at m/z [m + h-h(2)o](+), [m + h-2h(2)o](+), [m + h-3h(2)o](+), 347.3, and 290.1. repeatability was good for lc separation of baets-bile acid derivatives with aqueous acetonitrile as mobile phase. the relative standard deviations ( rsds) of retention time and peak area at 6.6 nmol ml(-1) levels with fluorescence detection (fl) were from 0.045 to 0.072% and from 2.16 to 2.73%, respectively. excellent linear responses were observed, with regression coefficients >0.9995. the fl detection limits (s/n=3) were in the range of 18.0-36.1 fmol. the online apci-ms detection limits are in the range of 500-790 fmol (at a signal-to-noise ratio of 3).
英文摘要	2-(5-benzoacridine)ethyl-p-toluenesulfonate (baets), a dual-sensitive probe, was reacted with bile acids in the presence of k(2)co(3) catalyst in dimethyl sulfoxide (dmso) solvent to give baets-bile acid derivatives. derivatives exhibited intense fluorescence (fl) with an excitation maximum lambda(ex) 270 nm and an emission maximum lambda(em) 510 nm. ms analysis using apci-ms indicated that derivatives had excellent apci-ms ionizability with percentage ionization delta values changing from 0 to 88.83% in aqueous acetonitrile and from 0 to 89.15% in aqueous methanol. the collision induced dissociation spectra of m/z [m + h](+) contained specific fragment ions at m/z [m + h-h(2)o](+), [m + h-2h(2)o](+), [m + h-3h(2)o](+), 347.3, and 290.1. repeatability was good for lc separation of baets-bile acid derivatives with aqueous acetonitrile as mobile phase. the relative standard deviations ( rsds) of retention time and peak area at 6.6 nmol ml(-1) levels with fluorescence detection (fl) were from 0.045 to 0.072% and from 2.16 to 2.73%, respectively. excellent linear responses were observed, with regression coefficients >0.9995. the fl detection limits (s/n=3) were in the range of 18.0-36.1 fmol. the online apci-ms detection limits are in the range of 500-790 fmol (at a signal-to-noise ratio of 3).
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; gas-chromatography ; human-serum ; precolumn derivatization ; biological-fluids ; silica cartridges ; carboxylic-acids ; fatty-acids ; column ; trifluoromethanesulfonate
收录类别	SCI ; ISTP
语种	英语
WOS记录号	WOS:000275752600030
公开日期	2011-12-13
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn//handle/363003/1726]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	You, Jinmao,Fu, Yanyan,Sun, Zhiwei,et al. 2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification[J]. analytical and bioanalytical chemistry,2010,396(7):2657-2666.
APA	You, Jinmao,Fu, Yanyan,Sun, Zhiwei,&Suo, Yourui.(2010).2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification.analytical and bioanalytical chemistry,396(7),2657-2666.
MLA	You, Jinmao,et al."2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification".analytical and bioanalytical chemistry 396.7(2010):2657-2666.