An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)

	An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)
	Hu, Yanping 1,2; Xie, Xiaolong 1,2; Wang, Li 1; Yang, Jian 1,2; Zhang, Huaigang 1; Li, Yi 1
刊名	african journal of biotechnology
	2009-06-17
卷号	8 期号:12 页码:2691-2694
关键词	Rhubarb Rheum tanguticum DNA extraction ISSR secondary metabolites
ISSN号	1684-5315
中文摘要	rhubarb is an important traditional chinese herbal drug with high secondary metabolites that interfere with dna extraction procedures and downstream applications, such as dna restriction and amplification. an effective and low-cost protocol for isolating genomic dna from root of rheum tanguticum is described in this paper. it involved a modified ctab method with distilled water pretreating samples. the a(260)/a(280) absorbance ratio of extracted dna, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. the dna preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (issr). this method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.
英文摘要	rhubarb is an important traditional chinese herbal drug with high secondary metabolites that interfere with dna extraction procedures and downstream applications, such as dna restriction and amplification. an effective and low-cost protocol for isolating genomic dna from root of rheum tanguticum is described in this paper. it involved a modified ctab method with distilled water pretreating samples. the a(260)/a(280) absorbance ratio of extracted dna, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. the dna preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (issr). this method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	conservation concern ; gene sequence ; pcr analysis ; identification ; optimization ; plants
收录类别	SCI
语种	英语
WOS记录号	WOS:000268831200006
公开日期	2011-12-13
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn//handle/363003/1856]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)[J]. african journal of biotechnology,2009,8(12):2691-2694.
APA	Hu, Yanping,Xie, Xiaolong,Wang, Li,Yang, Jian,Zhang, Huaigang,&Li, Yi.(2009).An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae).african journal of biotechnology,8(12),2691-2694.
MLA	Hu, Yanping,et al."An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)".african journal of biotechnology 8.12(2009):2691-2694.