| Mono-fullerenols modulating cell stiffness by perturbing actin bundling | |
| Gu, Weihong1,2; Bai, Xue1,2; Ren, Keli2,3; Zhao, Xiaoyi2,4; Xia, Shibo1,2; Zhang, Jiaxin1,2; Qin, Yanxia1; Lei, Runhong1; Chen, Kui1,2; Chang, Ya-nan1 | |
| 刊名 | Nanoscale
 |
| 2018-01-28 | |
| 卷号 | 10期号:4页码:1750-1758 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c7nr07231g |
| 通讯作者 | Xing, gengmei(xinggm@ihep.ac.cn) |
| 英文摘要 | Understanding what modulates the cell stiffness is important given its potential application as a diagnostic and medical target. here, we investigated why and how mono-fullerenols affect the cell stiffness. we confirmed the fullerenol-modulation of cell stiffness using atomic force microscopy (afm) with sphere tips and ascertained that the particles reduce the cell polarity. the structures of b-actin and f-actin were evaluated by inverted fluorescence microscopy, synchrotron radiation small angle x-ray scattering (saxs), transmission electron microscopy (tem) and afm. statistical and quantitative analyses of the saxs data of fullerenol-treated b-actin and f-actin reveal a transformation from large-size to small-size b-actin and simultaneously to f-actin. the slight increase in f-actin diameter in the treated group suggests that fullerenols attach to the actin surface. we verified the attachment using afm and high-resolution probes. collectively, our results suggest that fullerenols hamper the bundling of f-actin to form b-actin by adhering to the surface of f-actin, weakening the bundle-based cell stiffness. |
| WOS关键词 | BREAST-CANCER CELLS ; F-ACTIN ; STRUCTURAL FEATURES ; GOLD NANOPARTICLES ; LIPID-MEMBRANES ; FIBROBLASTS ; COLLAGEN ; POLARIZATION ; MIGRATION ; MECHANOTRANSDUCTION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000423355300022 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2177926 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Xing, Gengmei |
| 作者单位 | 1.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.UCAS, Beijing 100049, Peoples R China 3.Natl Ctr Nanosci & Technol NCNST, 11 Beiyitiao, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Weihong,Bai, Xue,Ren, Keli,et al. Mono-fullerenols modulating cell stiffness by perturbing actin bundling[J]. Nanoscale,2018,10(4):1750-1758. |
| APA | Gu, Weihong.,Bai, Xue.,Ren, Keli.,Zhao, Xiaoyi.,Xia, Shibo.,...&Xing, Gengmei.(2018).Mono-fullerenols modulating cell stiffness by perturbing actin bundling.Nanoscale,10(4),1750-1758. |
| MLA | Gu, Weihong,et al."Mono-fullerenols modulating cell stiffness by perturbing actin bundling".Nanoscale 10.4(2018):1750-1758. |
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