| Invasive fungi-derived defensins kill drug-resistant bacterial pathogens | |
| Wu, Jiajia; Liu, Shijie; Wang, Hao | |
| 刊名 | Peptides
 |
| 2018 | |
| 卷号 | 99页码:82-91 |
| 关键词 | Fungi Scedosporium Vancomycin-resistant enterococci Hydrophobicity |
| ISSN号 | 0196-9781 |
| DOI | 10.1016/j.peptides.2017.11.009 |
| 通讯作者 | Wang, hao(wanghao@nanoctr.cn) |
| 英文摘要 | Fungi-derived defensins are a class of antimicrobial peptides with therapeutic potential due to their high antibacterial efficacy and low toxicity. based on the genomic strategy, we have identified 68 fungal defensin-like peptides (fdlps) in five new genera, including trichosporon, apophysomyces, lichtheimia, beauveria and scedosporium and characterized a new synthetic defensin (scedosporisin) from an invasive fungus. it was active against gram-positive bacteria but not active against negative bacteria. importantly, it killed several clinical resistant isolates such as methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococci at low molecular concentrations. scedosporisin showed low hemolysis and cytotoxicity and high serum stability. the killing kinetics of scedosporisin-2 against a clinical isolate of mrsa showed that it killed the bacteria more rapidly than that of vancomycin. homology modeling analysis show that scedosporisin adopted a typical cysteine stabilized alpha-helical and beta-sheet fold with a local hydrophobic patch. scedosporisin significantly improved the survival rate of mice in the peritonitis model. this work has greatly expanded the library of fdlps, and successfully selected leading molecules for antimicrobial drug reserves. |
| WOS关键词 | ANTIMICROBIAL PEPTIDES ; ANTIBACTERIAL ACTIVITY ; ANTIBIOTIC-RESISTANCE ; DISCOVERY ; PLECTASIN ; EVOLUTION ; ALPHA ; STRATEGIES ; FAMILIES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Pharmacology & Pharmacy |
| WOS类目 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:000419525600011 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2177893 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wang, Hao |
| 作者单位 | Natl Ctr Nanosci & Technol NCNST, Lab Biol Effects Nanomat & Nanosafety, 11 Beiyitiao, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Jiajia,Liu, Shijie,Wang, Hao. Invasive fungi-derived defensins kill drug-resistant bacterial pathogens[J]. Peptides,2018,99:82-91. |
| APA | Wu, Jiajia,Liu, Shijie,&Wang, Hao.(2018).Invasive fungi-derived defensins kill drug-resistant bacterial pathogens.Peptides,99,82-91. |
| MLA | Wu, Jiajia,et al."Invasive fungi-derived defensins kill drug-resistant bacterial pathogens".Peptides 99(2018):82-91. |
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