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	Effects of fullerenol nanoparticles on rat oocyte meiosis resumption
	Lei, Runhong1; Bai, Xue1; Chang, Yanan1; Li, Juan1; Qin, Yanxia1; Chen, Kui1; Gu, Weihong1; Xia, Shibo1; Zhang, Jiaxin1; Wang, Zhenbo2
刊名	International journal of molecular sciences
	2018-03-01
卷号	19期号:3页码:15
关键词	Fullerenol Oocyte meiosis resumption Gap junction Granulosa cells Egfr/erk1/2
ISSN号	1422-0067
DOI	10.3390/ijms19030699
通讯作者	Wang, zhenbo(wangzb@ioz.ac.cn) ; Xing, gengmei(xinggm@ihep.ac.cn)
英文摘要	The excellent biocompatibility and biological effects of fullerenol and its derivatives make their biomedical application promising. the potential effects of fullerenol in mammals have been extensively studied, but little is known about its effects on female reproduction. using canonical oocyte-granulosa cell complexes (ogcs) in vitro maturation culture model, we investigated the effect of fullerenol on the first oocyte meiotic resumption. in the surrounding granulosa cells, fullerenol nanoparticles occluded the extracellular domain of the epidermal growth factor receptor (egfr) to reduce egfr-ligand binding and subsequent extracellular signal-regulated kinase 1 and 2 (erk1/2) activation, which involved the regulation of connexin 43 (cx43) expression and internalization. downregulation of cx43 expression and the retraction of transzonal projections (tzps) interrupted the gap junction channel and tzps based mass transportation. this effect decreased cyclic adenosine monophosphate (camp) levels in the oocyte and thereby accelerated rat oocyte meiosis resumption. moreover, perinuclear distribution of cx43 and egfr was observed in granulosa cells, which could further exacerbate the effects. fullerenol nanoparticles interfered with the strict process of oocyte meiosis resumption, which likely reduced the oocyte quality.
WOS关键词	TITANIUM-DIOXIDE NANOPARTICLES ; SILVER ALLOY NANOPARTICLES ; GAP-JUNCTION ENDOCYTOSIS ; REPRODUCTIVE TOXICITY ; MAMMALIAN OOCYTE ; DEVELOPMENTAL COMPETENCE ; MEIOTIC MATURATION ; GRANULOSA-CELLS ; GRAPHENE OXIDE ; GROWTH-FACTORS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
语种	英语
出版者	MDPI
WOS记录号	WOS:000428309800056
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2177515
专题	高能物理研究所
通讯作者	Wang, Zhenbo; Xing, Gengmei
作者单位	1.Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Lei, Runhong,Bai, Xue,Chang, Yanan,et al. Effects of fullerenol nanoparticles on rat oocyte meiosis resumption[J]. International journal of molecular sciences,2018,19(3):15.
APA	Lei, Runhong.,Bai, Xue.,Chang, Yanan.,Li, Juan.,Qin, Yanxia.,...&Xing, Gengmei.(2018).Effects of fullerenol nanoparticles on rat oocyte meiosis resumption.International journal of molecular sciences,19(3),15.
MLA	Lei, Runhong,et al."Effects of fullerenol nanoparticles on rat oocyte meiosis resumption".International journal of molecular sciences 19.3(2018):15.

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