| Reference channel-based microfluidic resistance sensing for single yeast cell volume growth measurement | |
| Sun, Jiashu1,2; Yang, Juekuan2,3,4; Gao, Yandong2; Xu, Dongyan5; Li, Deyu2 | |
| 刊名 | Microfluidics and nanofluidics
 |
| 2017-03-01 | |
| 卷号 | 21期号:3页码:9 |
| 关键词 | Resistance-channel based sensing Single-cell volume growth rate Yeast cell cycle |
| ISSN号 | 1613-4982 |
| DOI | 10.1007/s10404-017-1864-0 |
| 通讯作者 | Li, deyu(deyu.li@vanderbilt.edu) |
| 英文摘要 | A reference channel-based microfluidic sensing scheme for real-time measurements of volume growth rates of single cells has been demonstrated with single budding yeast. the microfluidic platform consists of two side-byside microchannels of the same geometry serving as the sensing channel and the reference channel, respectively, which allows for cancelation of the effects of baseline ionic current drift that commonly exists in microfluidic circuits. for a single cell growing inside the sensing channel, its volume growth rate is proportional to the resistance change in the sensing channel, which can be precisely measured in a continuous manner with the aid of the reference channel to effectively eliminate the fluctuations and drifts of the ionic current inside microfluidic channels. compared with the commonly used resistive-pulse sensing technique, the reported label-free sensing scheme can detect long-term volume growth rates of single cells attached to a substrate in real time, which is required for most mammalian cells to survive, without the involvement of expensive imaging instruments. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; REAL-TIME ; CYTOMETRY ; PLATFORM ; DEVICES ; CYCLE ; CHIP ; DIFFERENTIATION ; ELECTROLYSIS |
| WOS研究方向 | Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics |
| WOS类目 | Nanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Fluids & Plasmas |
| 语种 | 英语 |
| 出版者 | SPRINGER HEIDELBERG |
| WOS记录号 | WOS:000401005700004 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2177430 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Li, Deyu |
| 作者单位 | 1.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China 2.Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA 3.Southeast Univ, Sch Mech Engn, Nanjing 210096, Jiangsu, Peoples R China 4.Southeast Univ, Jiangsu Key Lab Design & Manufacture Micronano Bi, Nanjing 210096, Jiangsu, Peoples R China 5.Chinese Univ Hong Kong, Dept Mech & Automat Engn, Shatin, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jiashu,Yang, Juekuan,Gao, Yandong,et al. Reference channel-based microfluidic resistance sensing for single yeast cell volume growth measurement[J]. Microfluidics and nanofluidics,2017,21(3):9. |
| APA | Sun, Jiashu,Yang, Juekuan,Gao, Yandong,Xu, Dongyan,&Li, Deyu.(2017).Reference channel-based microfluidic resistance sensing for single yeast cell volume growth measurement.Microfluidics and nanofluidics,21(3),9. |
| MLA | Sun, Jiashu,et al."Reference channel-based microfluidic resistance sensing for single yeast cell volume growth measurement".Microfluidics and nanofluidics 21.3(2017):9. |
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