| Gene regulation with carbon-based sirna conjugates for cancer therapy | |
| Zhang, Lingmin; Zheng, Wenfu; Tang, Rongbing; Wang, Nuoxin; Zhang, Wei; Jiang, Xingyu | |
| 刊名 | Biomaterials
 |
| 2016-10-01 | |
| 卷号 | 104页码:269-278 |
| 关键词 | Fluorescent carbon nanoparticle Gene regulation Cancer therapy Plk1 gene Small interfering rna |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2016.07.015 |
| 通讯作者 | Jiang, xingyu(xingyujiang@nanoctr.cn) |
| 英文摘要 | We report fluorescent carbon nanoparticle (fcn)-based small interfering rna (sirna) conjugates (c-sirna) for gene regulation and cancer therapy. the c-sirna has a core of chitosan-derived fcn and a shell of sirna, and can down-regulate the expression of polo-like kinase-1 (plk1), a master regulator of mitosis, via sirna targeting plk1 (siplk1), for cancer therapy. the required amount of the fcns is only similar to 1/30 of that of the gold nanoparticles in delivering equal amount of sirna. the c-siplk1 led to similar to 80% knockdown of cellular plk1 mrna in a375 cells, and induced apoptosis of the a375 cells (31.9%) and mcf-7 cells (20.33%), much higher than those by commercial nonviral gene delivery vectors, such as lipofectamine 2000 in both cell lines (apoptosis rate < 10%). after the c-siplk1 was administrated to a375 tumor-bearing mice intravenously, the tumor volume was less than 1/11 of the control groups. the c-sirna can thus be powerful tools for gene delivery and gene therapy. (c) 2016 elsevier ltd. all rights reserved. |
| WOS关键词 | SPHERICAL NUCLEIC-ACIDS ; INTRACELLULAR TRAFFICKING ; IN-VITRO ; DELIVERY ; NANOPARTICLES ; VECTORS ; GRAPHENE ; DOTS ; THERAPEUTICS ; FLUORESCENCE |
| WOS研究方向 | Engineering ; Materials Science |
| WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000382345000024 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2176653 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Jiang, Xingyu |
| 作者单位 | Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lingmin,Zheng, Wenfu,Tang, Rongbing,et al. Gene regulation with carbon-based sirna conjugates for cancer therapy[J]. Biomaterials,2016,104:269-278. |
| APA | Zhang, Lingmin,Zheng, Wenfu,Tang, Rongbing,Wang, Nuoxin,Zhang, Wei,&Jiang, Xingyu.(2016).Gene regulation with carbon-based sirna conjugates for cancer therapy.Biomaterials,104,269-278. |
| MLA | Zhang, Lingmin,et al."Gene regulation with carbon-based sirna conjugates for cancer therapy".Biomaterials 104(2016):269-278. |
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