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	Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach
	Liu, Lei1; Li, Yuru1; Li, Ting1; Xie, Jiani1; Chen, Chaofei2; Liu, Quansheng2; Zhang, Shouwen3; Wu, Hai -Chen1
刊名	Analytical chemistry
	2016-01-19
卷号	88期号:2页码:1073-1077
ISSN号	0003-2700
DOI	10.1021/acs.analchem.5b04102
通讯作者	Liu, lei(leiliu@ihep.ac.cn) ; Wu, hai -chen(haichenwu@ihep.ac.cn)
英文摘要	We have developed a nanopore sensing approach for the selective detection of 8-oxo-2'-deoxyguanosine (8-oxog) in single-stranded dna. first, 1,12-dodecanediamine is coupled with 8-oxog-containing dna molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. after incubation with cucurbit[7]uril, the host-guest complex-modified dna hybrid is translocated through an alpha-hemolysin nanopore. highly characteristic events can be recorded and used to quantify the 8-oxog-dna content in a dna mixture. compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxog site in single-stranded dna at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples.
WOS关键词	ALPHA-HEMOLYSIN NANOPORE ; HOST-GUEST INTERACTIONS ; MOLECULE DETECTION ; OXIDATIVE DAMAGE ; COMET ASSAY ; PROTEIN ; 5-HYDROXYMETHYLCYTOSINE ; 8-OXOGUANINE ; PORE ; 8-OXO-2'-DEOXYGUANOSINE
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000368562300002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176570
专题	高能物理研究所
通讯作者	Liu, Lei; Wu, Hai -Chen
作者单位	1.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Multidisciplinary Ctr, Beijing 100049, Peoples R China 3.Beijing ChaoYang Emergency Med Ctr, Epilepsy Dept, Beijing 100021, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lei,Li, Yuru,Li, Ting,et al. Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach[J]. Analytical chemistry,2016,88(2):1073-1077.
APA	Liu, Lei.,Li, Yuru.,Li, Ting.,Xie, Jiani.,Chen, Chaofei.,...&Wu, Hai -Chen.(2016).Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach.Analytical chemistry,88(2),1073-1077.
MLA	Liu, Lei,et al."Selective detection of 8-oxo-2'-deoxyguanosine in single-stranded dna via nanopore sensing approach".Analytical chemistry 88.2(2016):1073-1077.

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