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	Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery
	Zhao, Caiyan; Shao, Leihou; Lu, Jianqing; Deng, Xiongwei; Wu, Yan
刊名	Acs applied materials & interfaces
	2016-03-16
卷号	8期号:10页码:6400-6410
关键词	Sheddable Tumor acidity Pegylation Polyethylenimine-poly(trimethylene carbonate) Gene delivery
ISSN号	1944-8244
DOI	10.1021/acsami.6b00825
通讯作者	Wu, yan(wuy@nanoctr.cn)
英文摘要	Amphiphilic pei derivatives/dna complexes are widely used for dna delivery, but they are unstable in vivo and have cytotoxicity due to the excess cationic charge. pegylation of cationic complexes can improve sterical stability and biocompatibility. however, pegylation significantly inhibits cellular uptake and endosomal escape. in this work, sheddable ternary complexes were developed by coating a tumor acidity-sensitive beta-carboxylic amide functionalized peg layer on the binary complexes of amphiphilic cationic polyethylenimine-poly(trimethylene carbonate) nanoparticles/dna (pei-ptmc/dna). such sheddable ternary complexes markedly reduced their nonspecific interactions with serum protein in the bloodstream and obtained minimal cytotoxicity due to the protection of the peg shell. at the tumor site, the peg layer was deshielded by responding to the tumor acidic microenvironment and the positively charged complexes re-exposed that had higher affinity with negatively charged cell membranes. meanwhile the positively charged complexes facilitated endosomal escape. accordingly, this delivery system improved the biocompatibility of gene-loaded complexes and enhanced the gene transfection efficiency. such pegylated complexes with the ability to deshield the peg layer at the target tissues hold great promise for efficient and safe gene delivery in vivo.
WOS关键词	TARGETED SIRNA DELIVERY ; POLY(TRIMETHYLENE CARBONATE) ; IN-VITRO ; CANCER-THERAPY ; DRUG-DELIVERY ; NANOPARTICLES ; PH ; POLYETHYLENIMINE ; TRANSFECTION ; COPOLYMERS
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000372479300013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176555
专题	高能物理研究所
通讯作者	Wu, Yan
作者单位	Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, 11 Beiyitiao, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Caiyan,Shao, Leihou,Lu, Jianqing,et al. Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery[J]. Acs applied materials & interfaces,2016,8(10):6400-6410.
APA	Zhao, Caiyan,Shao, Leihou,Lu, Jianqing,Deng, Xiongwei,&Wu, Yan.(2016).Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery.Acs applied materials & interfaces,8(10),6400-6410.
MLA	Zhao, Caiyan,et al."Tumor acidity-induced sheddable polyethylenimine-poly(trimethylene carbonate)/dna/polyethylene glycol-2,3-dimethylmaleicanhydride ternary complex for efficient and safe gene delivery".Acs applied materials & interfaces 8.10(2016):6400-6410.

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