| Porous silicon microparticles for delivery of sirna therapeutics | |
| Shen, Jianliang1,2; Wu, Xiaoyan1,3; Lee, Yeonju1; Wolfram, Joy1,4; Yang, Zhizhou1; Mao, Zong-Wan2; Ferrari, Mauro1,5; Shen, Haifa1,6 | |
| 刊名 | Jove-journal of visualized experiments
 |
| 2015 | |
| 期号 | 95页码:9 |
| 关键词 | Bioengineering Issue 95 Porous silicon Sirna Nanodelivery system Cancer therapy Transfection Polycation functionalization |
| ISSN号 | 1940-087X |
| DOI | 10.3791/52075 |
| 通讯作者 | Shen, haifa(hshen@houstonmethodist.org) |
| 英文摘要 | Small interfering rna (sirna) can be used to suppress gene expression, thereby providing a new avenue for the treatment of various diseases. however, the successful implementation of sirna therapy requires the use of delivery platforms that can overcome the major challenges of sirna delivery, such as enzymatic degradation, low intracellular uptake and lysosomal entrapment. here, a protocol for the preparation and use of a biocompatible and effective sirna delivery system is presented. this platform consists of polyethylenimine (pei) and arginine (arg)-grafted porous silicon microparticles, which can be loaded with sirna by performing a simple mixing step. the silicon particles are gradually degraded over time, thereby triggering the formation of arg-pei/sirna nanoparticles. this delivery vehicle provides a means for protecting and internalizing sirna, without causing cytotoxicity. the major steps of polycation functionalization, particle characterization, and sirna loading are outlined in detail. in addition, the procedures for determining particle uptake, cytotoxicity, and transfection efficacy are also described. |
| WOS关键词 | PARTICLES ; DESIGN |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | JOURNAL OF VISUALIZED EXPERIMENTS |
| WOS记录号 | WOS:000361532900021 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2176397 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Shen, Haifa |
| 作者单位 | 1.Houston Methodist Res Inst, Dept Nanomed, Houston, TX USA 2.Sun Yat Sen Univ, Sch Chem & Chem Engn, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou, Guangdong, Peoples R China 3.Huazhong Univ Sci & Technol, Union Hosp, Dept Pediat, Wuhan, Peoples R China 4.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing, Peoples R China 5.Weill Cornell Med Coll, Dept Med, New York, NY USA 6.Weill Cornell Med Coll, Dept Cell & Dev Biol, New York, NY USA |
| 推荐引用方式 GB/T 7714 | Shen, Jianliang,Wu, Xiaoyan,Lee, Yeonju,et al. Porous silicon microparticles for delivery of sirna therapeutics[J]. Jove-journal of visualized experiments,2015(95):9. |
| APA | Shen, Jianliang.,Wu, Xiaoyan.,Lee, Yeonju.,Wolfram, Joy.,Yang, Zhizhou.,...&Shen, Haifa.(2015).Porous silicon microparticles for delivery of sirna therapeutics.Jove-journal of visualized experiments(95),9. |
| MLA | Shen, Jianliang,et al."Porous silicon microparticles for delivery of sirna therapeutics".Jove-journal of visualized experiments .95(2015):9. |
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