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	Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment
	Ma, Xiaowei2,3; Wu, Yanyang1; Jin, Shubin2,3; Tian, Yuan5; Zhang, Xiaoning4; Zhao, Yuliang2,3; Yu, Li1; Liang, Xing-Jie2,3
刊名	Acs nano
	2011-11-01
卷号	5期号:11页码:8629-8639
关键词	Gold nanoparticles (aunps) Autophagosome accumulation Autophagic flux Lysosome impairment Lysosomal ph
ISSN号	1936-0851
DOI	10.1021/nn202155y
通讯作者	Yu, li(liyulab@mail.tsinghua.edu.cn)
英文摘要	Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. previous studies have shown that nanoparticles from various sources can induce autophagosome accumulation in treated cells. however, the underlying mechanism is still not clear. gold nanoparticles (aunps) are one of the most widely used nanomaterials and have been reported to induce autophagosome accumulation. in this study, we found that aunps can be taken into cells through endocytosis in a size-dependent manner. the internalized aunps eventually accumulate in lysosomes and cause impairment of lysosome degradation capacity through alkalinization of lysosomal ph. consistent with previous studies, we found that aunp treatment can induce autophagosome accumulation and processing of lc3, an autophagosome marker protein. however, degradation of the autophagy substrate p62 is blocked in aunp-treated cells, which indicates that autophagosome accumulation results from blockade of autophagy flux, rather than induction of autophagy. our data clarify the mechanism by which aunps induce autophagosome accumulation and reveal the effect of aunps on lysosomes. this work is significant to nanoparticle research because it illustrates how nanoparticles can potentially interrupt the autophagic pathway and has important implications for biomedical applications of nanoparticles.
WOS关键词	CELL-DEATH ; ENDOPLASMIC-RETICULUM ; QUANTUM DOTS ; ENDOCYTOSIS ; PH ; PACLITAXEL ; SURVIVAL ; MACROPHAGES ; METABOLISM ; ACTIVATION
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000297143300020
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176133
专题	高能物理研究所
通讯作者	Yu, Li
作者单位	1.Tsinghua Univ, Sch Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China 2.Natl Ctr Nanosci & Technol, Div Nanomed & Nanobiol, Lab Nanomed & Nanosafety, Beijing, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 4.Tsinghua Univ, Coll Med, Lab Pharmaceut, Beijing 100084, Peoples R China 5.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Ma, Xiaowei,Wu, Yanyang,Jin, Shubin,et al. Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment[J]. Acs nano,2011,5(11):8629-8639.
APA	Ma, Xiaowei.,Wu, Yanyang.,Jin, Shubin.,Tian, Yuan.,Zhang, Xiaoning.,...&Liang, Xing-Jie.(2011).Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment.Acs nano,5(11),8629-8639.
MLA	Ma, Xiaowei,et al."Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment".Acs nano 5.11(2011):8629-8639.

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