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	The mechanism of the molecular interaction between cerium (iii) and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco)
	Liu, Chao1,2; Hong, Fa-shui1,2; Tao, Ye3; Liu, Tao3; Xie, Ya-ning3; Xu, Jian-hua3; Li, Zhong-rui4
刊名	Biological trace element research
	2011-11-01
卷号	143期号:2页码:1110-1120
关键词	Rubisco Cerium Uv Fluorescence Exafs
ISSN号	0163-4984
DOI	10.1007/s12011-010-8888-6
通讯作者	Hong, fa-shui(hongfsh_cn@sina.com)
英文摘要	The mechanism of the molecular interaction between ce(3+), a member of rare earth elements, and rubisco in vitro is investigated. the carboxylase activity of rubisco greatly increased under low concentrations of ce(3+) and decreased under high concentrations of ce(3+). the ultraviolet absorption spectra show that the various concentrations of ce(3+) treatment do not shift the characteristic peaks of rubisco while the characteristic peak intensity of rubisco increases with increasing ce(3+) concentration. the rubisco-ce(3+) interactions also do not cause any noticeable change in the lambda max of rubisco fluorescence spectra. however, the fluorescence intensity of rubisco is found quenched by the addition of ce(3+), which strongly suggests that ce(3+) could directly bind to the rubisco protein. and the binding sites is estimated to 1.52 per protein. the binding between ce(3+) and rubisco is also proved by extended x-ray absorption fine-structure essay; ce(3+) coordinated with eight oxygen atoms of rubisco in first shells and six oxygen atoms in second shells. the results implied that ce(3+) might improve the microenvironment of rubisco and, in turn, affected the carboxylase capacity of rubisco greatly.
WOS关键词	RIBULOSE 1,5-BISPHOSPHATE ; WAVELET TRANSFORM ; SPINACH ; LANTHANUM ; OXYGENASE ; ACTIVASE ; BISPHOSPHATE ; CHLOROPHYLL ; TRANSITION ; PROTEIN
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
语种	英语
出版者	HUMANA PRESS INC
WOS记录号	WOS:000295609300050
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176120
专题	高能物理研究所
通讯作者	Hong, Fa-shui
作者单位	1.Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China 2.Soochow Univ, Coll Med, Suzhou 215123, Peoples R China 3.Chinese Acad Sci, Synchrotron Radiat Lab, Inst High Energy Phys, Beijing 100039, Peoples R China 4.Univ Oklahoma, Sch Chem Biol & Mat Engn, Norman, OK 73019 USA
推荐引用方式 GB/T 7714	Liu, Chao,Hong, Fa-shui,Tao, Ye,et al. The mechanism of the molecular interaction between cerium (iii) and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco)[J]. Biological trace element research,2011,143(2):1110-1120.
APA	Liu, Chao.,Hong, Fa-shui.,Tao, Ye.,Liu, Tao.,Xie, Ya-ning.,...&Li, Zhong-rui.(2011).The mechanism of the molecular interaction between cerium (iii) and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco).Biological trace element research,143(2),1110-1120.
MLA	Liu, Chao,et al."The mechanism of the molecular interaction between cerium (iii) and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco)".Biological trace element research 143.2(2011):1110-1120.

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