| Enhanced gene delivery and sirna silencing by gold nanoparticles coated with charge-reversal polyelectrolyte | |
| Guo, Shutao1,2,3; Huang, Yuanyu4; Jiang, Qiao1; Sun, Yun1; Deng, Liandong2,3; Liang, Zicai4; Du, Quan4; Xing, Jinfeng2,3; Zhao, Yuliang1; Wang, Paul C.1 | |
| 刊名 | Acs nano
 |
| 2010-09-01 | |
| 卷号 | 4期号:9页码:5505-5511 |
| 关键词 | Gold nanoparticles Charge-reversal polyelectrolyte Drug delivery Layer-by-layer assembly Sirna delivery |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/nn101638u |
| 通讯作者 | Liang, xing-jie(liangxj@nanoctr.cn) |
| 英文摘要 | Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering rna (sirna) and plasmid dna into cancer cells. polyacrylamide gel electrophoresis measurements of sirna confirmed the occurrence of the charge-reversal property of functional gold nanoparticles. the expression efficiency of enhanced green fluorescent protein (egfp) was improved by adjuvant transfection with charge-reversal functional gold nanoparticles, which also had much lower toxicity to cell proliferation. lamin a/c, an important nuclear envelope protein, was effectively silenced by lamin a/c-sirna delivered by charge-reversal functional gold nanoparticles, whose knockdown efficiency was better than that of commercial lipofectamine 2000. confocal laser scanning microscopic images indicated that there was more cy5-sirna distributed throughout the cytoplasm for cyanine 5-sirna/polyethyleneimine/cis-aconitic anhydride-functionalized poly(allylamine)/polyethyleneimine/11-mercaptoundecanoic acid-gold nanoparticle (cy5-sirna/pei/pah-cit/pei/mua-aunp) complexes. these results demonstrate the feasibility of using charge-reversal functional gold nanoparticles as a means of improving the nucleic acid delivery efficiency. |
| WOS关键词 | DRUG-DELIVERY ; RELEASE ; EFFICIENT ; CELLS ; DNA ; TRANSFECTION ; DISRUPTION ; MONOLAYER |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000282121000068 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2175994 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Liang, Xing-Jie |
| 作者单位 | 1.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China 3.Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300072, Peoples R China 4.Peking Univ, Inst Mol Med, Lab Nucle Acid Technol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Shutao,Huang, Yuanyu,Jiang, Qiao,et al. Enhanced gene delivery and sirna silencing by gold nanoparticles coated with charge-reversal polyelectrolyte[J]. Acs nano,2010,4(9):5505-5511. |
| APA | Guo, Shutao.,Huang, Yuanyu.,Jiang, Qiao.,Sun, Yun.,Deng, Liandong.,...&Liang, Xing-Jie.(2010).Enhanced gene delivery and sirna silencing by gold nanoparticles coated with charge-reversal polyelectrolyte.Acs nano,4(9),5505-5511. |
| MLA | Guo, Shutao,et al."Enhanced gene delivery and sirna silencing by gold nanoparticles coated with charge-reversal polyelectrolyte".Acs nano 4.9(2010):5505-5511. |
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