Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate
	Ding, JW ; Qin, W
刊名	ELECTROANALYSIS
	2009
卷号	21 期号:17-18 页码:2030-2035
关键词	Polymer membrane electrodes Controlled-release Butyrylcholinesterase Pesticides Biosensors Membranes
ISSN号	1040-0397
通讯作者	wqin@yic.ac.cn
中文摘要	A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).
学科主题	Chemistry, Analytical; Electrochemistry
收录类别	SCI
原文出处	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000270399100020
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/6165]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Ding, JW,Qin, W. Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate[J]. ELECTROANALYSIS,2009,21(17-18):2030-2035.
APA	Ding, JW,&Qin, W.(2009).Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate.ELECTROANALYSIS,21(17-18),2030-2035.
MLA	Ding, JW,et al."Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate".ELECTROANALYSIS 21.17-18(2009):2030-2035.