Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence | |
Zhang, JB ; Huang, H ; Cai, ZP ; Huang, LM | |
刊名 | FOOD CONTROL |
2006 | |
卷号 | 17期号:7页码:557-563 |
关键词 | identification red snappers semi-nested PCR 12S rRNA gene |
ISSN号 | 0956-7135 |
通讯作者 | jbzhang30@sina.com.cn |
中文摘要 | A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved. |
学科主题 | Food Science & Technology |
收录类别 | SCI |
原文出处 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000235266600009 |
公开日期 | 2011-07-03 |
内容类型 | 期刊论文 |
源URL | [http://ir.scsio.ac.cn/handle/344004/5817] |
专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
推荐引用方式 GB/T 7714 | Zhang, JB,Huang, H,Cai, ZP,et al. Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence[J]. FOOD CONTROL,2006,17(7):557-563. |
APA | Zhang, JB,Huang, H,Cai, ZP,&Huang, LM.(2006).Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence.FOOD CONTROL,17(7),557-563. |
MLA | Zhang, JB,et al."Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence".FOOD CONTROL 17.7(2006):557-563. |
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