Biochemical pathway and degradation of phthalate ester isomers by bacteria

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Biochemical pathway and degradation of phthalate ester isomers by bacteria
	Gu, JD ; Li, J ; Wang, Y
刊名	WATER SCIENCE AND TECHNOLOGY
	2005
卷号	52 期号:8 页码:241-248
关键词	biochemical cooperation degradation phthalate ester plasticizer wetland
ISSN号	0273-1223
通讯作者	jdgu@hkucc.hku.hk
中文摘要	Degradation of dimethyl isophthalate (DMI) and dimethyl phthalate ester (DMPE) was investigated using microorganisms isolated from mangrove sediment of Hong Kong Mai Po Nature Reserve. One enrichment culture was capable of utilizing DMI as the sole source of carbon and energy, but none of the bacteria in the enrichment culture was capable of degrading DMI alone. In co-culture of two bacteria, degradation was observed proceeding through monomethyl isophthalate (MMI) ester and isophthalic acid (IPA) before the aromatic ring opening. Using DMI as the sole carbon and energy source, Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr degraded DMI through the biochemical cooperation. The initial hydrolytic reaction of the ester bond was by K oxytoca Sc and the next step of transformation was by M. mesophilicum Sr, and IPA was degraded by both of them. In another investigation, a novel bacterium, strain MPsc, was isolated for degradation of dimethyl phthalate ester (DMPE) also from the mangrove sediment. On the basis of phenotypic, biochemical and 16S rDNA gene sequence analyses, the strain MPsc should be considered as a new bacterium at the genus level (8% differences). This strain, together with a Rhodococcus zopfii isolated from the same mangrove sediment, was able to degrade DMPE aerobically. The consortium consisting of the two species degraded 450 mg/l DMPE within 3 days as the sole source of carbon and energy, but none of the individual species alone was able to transform DMPE. Furthermore, the biochemical degradation pathway proceeded through monomethyl phthalate (MMP), phthalic acid (PA) and then protocatechuate before aromatic ring cleavage. Our results suggest that degradation of complex organic compounds including DMI and DMPE may be carried out by several members of microorganisms working together in the natural environments.
学科主题	Engineering, Environmental; Environmental Sciences; Water Resources
收录类别	SCI
WOS记录号	WOS:000233644300029
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5687]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Gu, JD,Li, J,Wang, Y. Biochemical pathway and degradation of phthalate ester isomers by bacteria[J]. WATER SCIENCE AND TECHNOLOGY,2005,52(8):241-248.
APA	Gu, JD,Li, J,&Wang, Y.(2005).Biochemical pathway and degradation of phthalate ester isomers by bacteria.WATER SCIENCE AND TECHNOLOGY,52(8),241-248.
MLA	Gu, JD,et al."Biochemical pathway and degradation of phthalate ester isomers by bacteria".WATER SCIENCE AND TECHNOLOGY 52.8(2005):241-248.