Rapid growth and sterility of growth hormone gene transgenic triploid carp

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Rapid growth and sterility of growth hormone gene transgenic triploid carp
	Yu Fan 1; Xiao Jun 1; Liang XiangYang 1; Liu ShaoJun 1; Zhou GongJian 1; Luo KaiKun 1; Liu Yun 1; Hu Wei 2; Wang YaPing 2; Zhu ZuoYan 2
刊名	CHINESE SCIENCE BULLETIN
	2011-06-01
卷号	56 期号:16 页码:1679-1684
关键词	transgene carp diploid triploid improved tetraploid hybrids of red crucian carpxcommon carp sterility ecological safety
ISSN号	1001-6538
通讯作者	Liu, SJ, Hunan Normal Univ, Coll Life Sci, Educ Minist China, Key Lab Prot Chem & Fish Dev Biol, Changsha 410081, Hunan, Peoples R China ; lsj@hunnu.edu.cn ; zhuzy@nsfc.gov.cn
中文摘要	Triploid carp (100%) with 150 (3n=150) chromosomes were obtained by crossing the females of improved tetraploid hybrids (a (TM) Euro, 4n=200) of red crucian carp (a (TM) Euro)xcommon carp (a (TM),) with the males of diploid yellow river carp (a (TM),, 2n=100). The crosses yielded transgenic triploid carp (positive triploid fish, 44.2% of the progeny) and non-transgenic triploid carp (negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season. Morphologically, both the positive and negative triploid fish were similar. They had a spindle-shaped, laterally compressed, steel grey body with two pairs of barbells. Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents. The positive and negative triploid fish were raised in the same pond for 2 years. The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish. The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish. Thus, we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.
英文摘要	Triploid carp (100%) with 150 (3n=150) chromosomes were obtained by crossing the females of improved tetraploid hybrids (a (TM) Euro, 4n=200) of red crucian carp (a (TM) Euro)xcommon carp (a (TM),) with the males of diploid yellow river carp (a (TM),, 2n=100). The crosses yielded transgenic triploid carp (positive triploid fish, 44.2% of the progeny) and non-transgenic triploid carp (negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season. Morphologically, both the positive and negative triploid fish were similar. They had a spindle-shaped, laterally compressed, steel grey body with two pairs of barbells. Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents. The positive and negative triploid fish were raised in the same pond for 2 years. The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish. The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish. Thus, we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	RED CRUCIAN CARP ; X COMMON CARP ; ATLANTIC SALMON ; FISH ; HYBRIDIZATION ; HYBRIDS
收录类别	SCI
资助信息	National Key Basic Research Program of China [2007CB109206]; National Special Fund for Research in Public Welfare Sector [200903046-08]
语种	英语
WOS记录号	WOS:000290799600007
公开日期	2011-06-30
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15640]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Hunan Normal Univ, Coll Life Sci, Educ Minist China, Key Lab Prot Chem & Fish Dev Biol, Changsha 410081, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Yu Fan,Xiao Jun,Liang XiangYang,et al. Rapid growth and sterility of growth hormone gene transgenic triploid carp[J]. CHINESE SCIENCE BULLETIN,2011,56(16):1679-1684.
APA	Yu Fan.,Xiao Jun.,Liang XiangYang.,Liu ShaoJun.,Zhou GongJian.,...&Zhu ZuoYan.(2011).Rapid growth and sterility of growth hormone gene transgenic triploid carp.CHINESE SCIENCE BULLETIN,56(16),1679-1684.
MLA	Yu Fan,et al."Rapid growth and sterility of growth hormone gene transgenic triploid carp".CHINESE SCIENCE BULLETIN 56.16(2011):1679-1684.