Acute and chronic un-ionized ammonia toxicity to 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)

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	Acute and chronic un-ionized ammonia toxicity to 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)
	Guan Bo 1,2; Hu Wei 1; Zhang TangLin 1; Duan Ming 1,2; Li DeLiang 1,2; Wang YaPing 1; Zhu ZuoYan 1
刊名	CHINESE SCIENCE BULLETIN
	2010-12-01
卷号	55 期号:35 页码:4032-4036
关键词	transgenic common carp growth hormone un-ionized ammonia toxicity mortality
ISSN号	1001-6538
通讯作者	Hu, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; huwei@ihb.ac.cn
中文摘要	Ammonia is toxic to fish in natural and artificial waters. We evaluated the acute (96 h) and chronic (21 d) toxicity of un-ionized ammonia to GH transgenic common carp (Cyprinus carpio L.) and non-transgenic common carp using a static-renewal bioassay. The 24, 48, 72 and 96 h median lethal concentrations (LC50) of un-ionized ammonia were slightly lower in transgenic carp (2.64, 2.44, 2.28 and 2.16 mg N/L, respectively) than in non-transgenic carp (2.70, 2.64, 2.52 and 2.33 mg N/L, respectively). Similarly, the median lethal time (LT50) was significantly shorter for transgenic carp (1.41, 7.91 and 117.42 h) than for non-transgenic common carp (2.53, 14.06 and 150.44 h) following exposure to 3.86, 3.29, or 2.09 mg N/L, respectively. Moreover, the mortality of transgenic carp was significantly higher than that of non-transgenic carp at all un-ionized ammonia concentrations ((0.91 +/- 0.12), (0.48 +/- 0.06) and (0.12 +/- 0.01) mg N/L) during the 21 d chronic toxicity test. Our results suggest that GH transgenic carp are less tolerant of un-ionized ammonia than non-transgenic carp. Our data are useful for evaluating potential environmental risk, optimizing stocking density in intensive aquaculture and establishing water quality criteria for ammonia in aquaculture.
英文摘要	Ammonia is toxic to fish in natural and artificial waters. We evaluated the acute (96 h) and chronic (21 d) toxicity of un-ionized ammonia to GH transgenic common carp (Cyprinus carpio L.) and non-transgenic common carp using a static-renewal bioassay. The 24, 48, 72 and 96 h median lethal concentrations (LC(50)) of un-ionized ammonia were slightly lower in transgenic carp (2.64, 2.44, 2.28 and 2.16 mg N/L, respectively) than in non-transgenic carp (2.70, 2.64, 2.52 and 2.33 mg N/L, respectively). Similarly, the median lethal time (LT(50)) was significantly shorter for transgenic carp (1.41, 7.91 and 117.42 h) than for non-transgenic common carp (2.53, 14.06 and 150.44 h) following exposure to 3.86, 3.29, or 2.09 mg N/L, respectively. Moreover, the mortality of transgenic carp was significantly higher than that of non-transgenic carp at all un-ionized ammonia concentrations ((0.91 +/- 0.12), (0.48 +/- 0.06) and (0.12 +/- 0.01) mg N/L) during the 21 d chronic toxicity test. Our results suggest that GH transgenic carp are less tolerant of un-ionized ammonia than non-transgenic carp. Our data are useful for evaluating potential environmental risk, optimizing stocking density in intensive aquaculture and establishing water quality criteria for ammonia in aquaculture.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	TILAPIA OREOCHROMIS-NILOTICUS ; ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; RECEPTOR ; EXPOSURE ; RAT ; SUSCEPTIBILITY ; TRANSCRIPTS ; PERFORMANCE ; EXPRESSION
收录类别	SCI
资助信息	National Basic Research Program of China [2007CB109205, 2009CB118804]; National Natural Science Foundation of China [30623001]
语种	英语
WOS记录号	WOS:000285196000009
公开日期	2011-06-30
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15634]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guan Bo,Hu Wei,Zhang TangLin,et al. Acute and chronic un-ionized ammonia toxicity to 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)[J]. CHINESE SCIENCE BULLETIN,2010,55(35):4032-4036.
APA	Guan Bo.,Hu Wei.,Zhang TangLin.,Duan Ming.,Li DeLiang.,...&Zhu ZuoYan.(2010).Acute and chronic un-ionized ammonia toxicity to 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.).CHINESE SCIENCE BULLETIN,55(35),4032-4036.
MLA	Guan Bo,et al."Acute and chronic un-ionized ammonia toxicity to 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)".CHINESE SCIENCE BULLETIN 55.35(2010):4032-4036.