Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein

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	Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein
	Guan, Ruizhang 1,2,3; Xiong, Jing 1,2,3; Huang, Wenshu 2; Guo, Songlin 2
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2011
卷号	43 期号:1 页码:79-88
关键词	outer membrane protein immunogenicity Aeromonas sobria Aeromonas hydrophila Anguilla anguilla
ISSN号	1672-9145
通讯作者	Guan, RZ, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; rzguan@jmu.edu.cn
中文摘要	To develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (OMP) antigen from different bacterial pathogens. In this study, an OMP fragment of 747 bp (named as Omp-G), which was highly conserved in seven Aeromonas OMP sequences from the NCBI database, was amplified by PCR from one Aeromonas sobria strain (B10) and two Aeromonas hydrophila strains (B27 and B33) with the designed specific primers. The sequence was cloned into pGEX-2T (6 x His-tag) vector, expressed in Escherichia coli system, and then the recombinant protein (named as rOmp-G) was purified with nickel chelating affinity chromatography. The purified rOmp-G showed a good immunogenicity in rabbits and well-conserved characteristics in these three pathogens by enzyme-linked immunosorbed assay. Furthermore, the rOmp-G also showed good immunogenicity in eels (Anguilla anguilla) for eliciting significantly increased specific antibodies (P < 0.01), and providing higher protection efficiencies (P < 0.05) after the pathogens challenge. The values of the relative percent survival in eels were 70% and 50% for two A. hydrophila strain challenge, and 75% for A. sobria strain challenge. This is the first report of a potential vaccination in eels that simultaneously provide protectiveness against different Aeromonas pathogens with a conserved partial OMP.
英文摘要	To develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (OMP) antigen from different bacterial pathogens. In this study, an OMP fragment of 747 bp (named as Omp-G), which was highly conserved in seven Aeromonas OMP sequences from the NCBI database, was amplified by PCR from one Aeromonas sobria strain (B10) and two Aeromonas hydrophila strains (B27 and B33) with the designed specific primers. The sequence was cloned into pGEX-2T (6 x His-tag) vector, expressed in Escherichia coli system, and then the recombinant protein (named as rOmp-G) was purified with nickel chelating affinity chromatography. The purified rOmp-G showed a good immunogenicity in rabbits and well-conserved characteristics in these three pathogens by enzyme-linked immunosorbed assay. Furthermore, the rOmp-G also showed good immunogenicity in eels (Anguilla anguilla) for eliciting significantly increased specific antibodies (P < 0.01), and providing higher protection efficiencies (P < 0.05) after the pathogens challenge. The values of the relative percent survival in eels were 70% and 50% for two A. hydrophila strain challenge, and 75% for A. sobria strain challenge. This is the first report of a potential vaccination in eels that simultaneously provide protectiveness against different Aeromonas pathogens with a conserved partial OMP.
学科主题	Biochemistry & Molecular Biology; Biophysics
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	EFFECTIVE VACCINE CANDIDATE ; EDWARDSIELLA-TARDA ; ATLANTIC SALMON ; VIBRIO-HARVEYI ; RAINBOW-TROUT ; MAJOR ADHESIN ; CLONING ; EXPRESSION ; VERONII ; FISH
收录类别	SCI
资助信息	Ministry of Agriculture of China [eel 3-51]; National Natural Science Foundation of China [30700625]; Department of Science and Technology of Fujian Province [2002N024]; Bureau of Science and Technology of Xiamen City [3502Z2001244]
语种	英语
WOS记录号	WOS:000285628600011
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15504]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Jimei Univ, Fishery Coll, Xiamen 361021, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Guan, Ruizhang,Xiong, Jing,Huang, Wenshu,et al. Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(1):79-88.
APA	Guan, Ruizhang,Xiong, Jing,Huang, Wenshu,&Guo, Songlin.(2011).Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(1),79-88.
MLA	Guan, Ruizhang,et al."Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.1(2011):79-88.