Antibacterial and Antiviral Roles of a Fish beta-Defensin Expressed Both in Pituitary and Testis

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	Antibacterial and Antiviral Roles of a Fish beta-Defensin Expressed Both in Pituitary and Testis
	Jin, Jun-Yan; Zhou, Li; Wang, Yang; Li, Zhi; Zhao, Jiu-Gang; Zhang, Qi-Ya; Gui, Jian-Fang
刊名	PLOS ONE
	2010-12-20
卷号	5 期号:12 页码:-e12883
ISSN号	1932-6203
通讯作者	Jin, JY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan, Peoples R China ; jfgui@ihb.ac.cn
中文摘要	Defensins are a group of cationic peptides that exhibit broad-spectrum antimicrobial activity. In this study, we cloned and characterized a beta-defensin from pituitary cDNA library of a protogynous hermaphroditic orange- spotted grouper (Epinephelus coioides). Interestingly, the beta-defensin was shown to be dominantly expressed in pituitary and testis by RT-PCR and Western blot analysis, and its transcript level is significantly upregulated in reproduction organs from intersexual gonad to testis during the natural and artificial sex reversal. Promoter sequence and the responsible activity region analyses revealed the pituitary-specific POU1F1a transcription binding site and testis-specific SRY responsible site, and demonstrated that the pituitary-specific POU1F1a transcription binding site that locates between 2180 and 2208 bp is the major responsible region of grouper beta-defensin promoter activity. Immunofluorescence localization observed its pituicyte expression in pituitary and spermatogonic cell expression in testis. Moreover, both in vitro antibacterial activity assay of the recombinant beta-defensin and in vivo embryo microinjection of the beta-defensin mRNA were shown to be effective in killing Gram-negative bacteria. And, its antiviral role was also demonstrated in EPC cells transfected with the beta-defensin construct. Additionally, the antibacterial activity was sensitive to concentrations of Na+, K+, Ca2+ and Mg2+. The above intriguing findings strongly suggest that the fish beta-defensin might play significant roles in both innate immunity defense and reproduction endocrine regulation.
英文摘要	Defensins are a group of cationic peptides that exhibit broad-spectrum antimicrobial activity. In this study, we cloned and characterized a beta-defensin from pituitary cDNA library of a protogynous hermaphroditic orange- spotted grouper (Epinephelus coioides). Interestingly, the beta-defensin was shown to be dominantly expressed in pituitary and testis by RT-PCR and Western blot analysis, and its transcript level is significantly upregulated in reproduction organs from intersexual gonad to testis during the natural and artificial sex reversal. Promoter sequence and the responsible activity region analyses revealed the pituitary-specific POU1F1a transcription binding site and testis-specific SRY responsible site, and demonstrated that the pituitary-specific POU1F1a transcription binding site that locates between 2180 and 2208 bp is the major responsible region of grouper beta-defensin promoter activity. Immunofluorescence localization observed its pituicyte expression in pituitary and spermatogonic cell expression in testis. Moreover, both in vitro antibacterial activity assay of the recombinant beta-defensin and in vivo embryo microinjection of the beta-defensin mRNA were shown to be effective in killing Gram-negative bacteria. And, its antiviral role was also demonstrated in EPC cells transfected with the beta-defensin construct. Additionally, the antibacterial activity was sensitive to concentrations of Na(+), K(+), Ca(2+) and Mg(2+). The above intriguing findings strongly suggest that the fish beta-defensin might play significant roles in both innate immunity defense and reproduction endocrine regulation.
学科主题	Biology; Multidisciplinary Sciences
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	NF-KAPPA-B ; ANTIMICROBIAL PEPTIDE ; DIFFERENTIAL EXPRESSION ; SEX REVERSAL ; HUMAN BETA-DEFENSIN-3 ; EPINEPHELUS-COIOIDES ; TRANSCRIPTION FACTOR ; REPRODUCTIVE-TRACT ; PLANT DEFENSINS ; SPOTTED GROUPER
收录类别	SCI
资助信息	National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [U0631007]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15, 085B181F01]; Institute of Hydrobiology, Chinese Academy of Sciences [075A011301, 085A011301]
语种	英语
WOS记录号	WOS:000285575200001
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15496]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Jin, Jun-Yan,Zhou, Li,Wang, Yang,et al. Antibacterial and Antiviral Roles of a Fish beta-Defensin Expressed Both in Pituitary and Testis[J]. PLOS ONE,2010,5(12):-e12883.
APA	Jin, Jun-Yan.,Zhou, Li.,Wang, Yang.,Li, Zhi.,Zhao, Jiu-Gang.,...&Gui, Jian-Fang.(2010).Antibacterial and Antiviral Roles of a Fish beta-Defensin Expressed Both in Pituitary and Testis.PLOS ONE,5(12),-e12883.
MLA	Jin, Jun-Yan,et al."Antibacterial and Antiviral Roles of a Fish beta-Defensin Expressed Both in Pituitary and Testis".PLOS ONE 5.12(2010):-e12883.