The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)

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	The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)
	Li, Deliang 1,2; Fu, Cuizhang 1,3; Wang, Yaping 1; Zhu, Zuoyan 1; Hu, Wei 1
刊名	AQUACULTURE
	2011-02-03
卷号	311 期号:1-4 页码:263-268
关键词	Common carp Transgenic Exhaustive exercise Growth hormone (GH) Hematology
ISSN号	0044-8486
通讯作者	Fu, CZ, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; czfu@fudan.edu.cn, huwei@ihb.ac.cn
中文摘要	This study examined the hematological profiles and responses to exhaustive exercise in 'all-fish' growth hormone (GH) transgenic common carp (Cyprinus carpio L) compared to non-transgenic controls. Transgenic fish had significantly smaller erythrocytes (length, width, surface area and volume), with a larger surface to volume ratios than non-transgenics. There were no significant differences in hematocrit, blood hemoglobin concentrations, plasma glucose, lactate, cholesterol and protein concentrations between the two strains at rest, but the transgenic fish had significantly lower resting mean corpuscular hemoglobin concentrations (MCHC) or higher plasma triglyceride concentrations than controls. Exhaustive exercise did not induce significant changes in hematocrit, hemoglobin concentrations and MCHC in either strain. The patterns of elevation and recovery in plasma glucose and lactate were the same for transgenic and control fish, although lactate levels were lower in the transgnic fish than controls at 1 h and 2 h post-exercise. There was a significant elevation in plasma cholesterol and triglyceride concentrations for 1 h post-exercise in transgenics but not in non-transgenic controls. Plasma protein concentrations were not altered by exhaustive exercise in transgenics but were lower from 2 h post-exercise in non-transgenic controls. These findings suggest that the 'all-fish' GH transgene significantly affects the erythrocyte morphology and the patterns in hematological response to exhaustive exercise of common carp, especially for lipid metabolism. The greater reliance on triglycerides and a greater erythrocyte surface to volume ratio may help transgenic carp to recover from exhaustive exercise, but further research on muscle metabolism and aerobic metabolic rate are needed in order to confirm this conclusion. (C) 2010 Elsevier B.V. All rights reserved.
英文摘要	This study examined the hematological profiles and responses to exhaustive exercise in 'all-fish' growth hormone (GH) transgenic common carp (Cyprinus carpio L) compared to non-transgenic controls. Transgenic fish had significantly smaller erythrocytes (length, width, surface area and volume), with a larger surface to volume ratios than non-transgenics. There were no significant differences in hematocrit, blood hemoglobin concentrations, plasma glucose, lactate, cholesterol and protein concentrations between the two strains at rest, but the transgenic fish had significantly lower resting mean corpuscular hemoglobin concentrations (MCHC) or higher plasma triglyceride concentrations than controls. Exhaustive exercise did not induce significant changes in hematocrit, hemoglobin concentrations and MCHC in either strain. The patterns of elevation and recovery in plasma glucose and lactate were the same for transgenic and control fish, although lactate levels were lower in the transgnic fish than controls at 1 h and 2 h post-exercise. There was a significant elevation in plasma cholesterol and triglyceride concentrations for 1 h post-exercise in transgenics but not in non-transgenic controls. Plasma protein concentrations were not altered by exhaustive exercise in transgenics but were lower from 2 h post-exercise in non-transgenic controls. These findings suggest that the 'all-fish' GH transgene significantly affects the erythrocyte morphology and the patterns in hematological response to exhaustive exercise of common carp, especially for lipid metabolism. The greater reliance on triglycerides and a greater erythrocyte surface to volume ratio may help transgenic carp to recover from exhaustive exercise, but further research on muscle metabolism and aerobic metabolic rate are needed in order to confirm this conclusion. (C) 2010 Elsevier B.V. All rights reserved.
学科主题	Fisheries; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	RAINBOW-TROUT ; WHITE MUSCLE ; ACID-BASE ; METABOLISM ; STRESS ; CORTISOL ; RECOVERY ; BLOOD ; STRAINS ; VOLUME
收录类别	SCI
资助信息	Development Plan of the State Key Fundamental Research of China [2007CB109205, 2009CB118804]; National Natural Science Foundation [30930069]
语种	英语
WOS记录号	WOS:000287432600039
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15488]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China 3.Fudan Univ, Inst Biodivers Sci, Key Lab Biodivers Sci & Ecol Engn, Minist Educ, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Li, Deliang,Fu, Cuizhang,Wang, Yaping,et al. The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)[J]. AQUACULTURE,2011,311(1-4):263-268.
APA	Li, Deliang,Fu, Cuizhang,Wang, Yaping,Zhu, Zuoyan,&Hu, Wei.(2011).The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.).AQUACULTURE,311(1-4),263-268.
MLA	Li, Deliang,et al."The hematological response to exhaustive exercise in 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)".AQUACULTURE 311.1-4(2011):263-268.