Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus

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	Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus
	Jia, W. Z.1,2; Shang, N.1; Guo, Q. L.1
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2010-09-01
卷号	36 期号:3 页码:451-459
关键词	Rhamnose-binding lectin Carbohydrate recognition domain Gene organization Promoter Snakehead Channa argus
ISSN号	0920-1742
通讯作者	Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Lectins are sugar-binding proteins that mediate pathogen recognition and cell-cell interactions. A rhamnose-binding lectin (RBL) gene and its promoter region have been cloned and characterized from snakehead Channa argus. From the transcription initiation site, snakehead rhamnose-binding lectin (SHL) gene extends 2,382 bp to the end of the 3' untranslated region (UTR), and contains nine exons and eight introns. The open reading frame (ORF) of the SHL transcript has 675 bp which encodes 224 amino acids. The molecular structure of SHL is composed of two tandem repeat carbohydrate recognition domains (CRD) with 35% internal identity. Analysis of the gene organization of SHL indicates that the ancestral gene of RBL may diverge and evolve by exon shuffling and gene duplication, producing new forms to play their own roles in various organisms. The characteristics of SHL gene 5' flanking region are the presence of consensus nuclear factor of interleukin 6 (NF-IL6) and IFN-gamma activation (GAS) sites. The results provide indirect evidence that up-regulation of SHL expression may be induced in response to inflammatory stimuli, such as lipopolysaccharide (LPS), interleukin 6 (IL-6), and interferon gamma (IFN-gamma). The transcript of SHL mRNA was expressed in the head kidney, posterior kidney, spleen, liver, intestine, heart, muscle, and ovary. No tissue-specific expressive pattern is different from reported STLs, WCLs, and PFLs, suggesting that different types of RBLs exist in species-specific fish that have evolved and adapted to their surroundings.
英文摘要	Lectins are sugar-binding proteins that mediate pathogen recognition and cell-cell interactions. A rhamnose-binding lectin (RBL) gene and its promoter region have been cloned and characterized from snakehead Channa argus. From the transcription initiation site, snakehead rhamnose-binding lectin (SHL) gene extends 2,382 bp to the end of the 3' untranslated region (UTR), and contains nine exons and eight introns. The open reading frame (ORF) of the SHL transcript has 675 bp which encodes 224 amino acids. The molecular structure of SHL is composed of two tandem repeat carbohydrate recognition domains (CRD) with 35% internal identity. Analysis of the gene organization of SHL indicates that the ancestral gene of RBL may diverge and evolve by exon shuffling and gene duplication, producing new forms to play their own roles in various organisms. The characteristics of SHL gene 5' flanking region are the presence of consensus nuclear factor of interleukin 6 (NF-IL6) and IFN-gamma activation (GAS) sites. The results provide indirect evidence that up-regulation of SHL expression may be induced in response to inflammatory stimuli, such as lipopolysaccharide (LPS), interleukin 6 (IL-6), and interferon gamma (IFN-gamma). The transcript of SHL mRNA was expressed in the head kidney, posterior kidney, spleen, liver, intestine, heart, muscle, and ovary. No tissue-specific expressive pattern is different from reported STLs, WCLs, and PFLs, suggesting that different types of RBLs exist in species-specific fish that have evolved and adapted to their surroundings.
学科主题	Biochemistry & Molecular Biology; Fisheries; Physiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Fisheries ; Physiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Fisheries ; Physiology
关键词[WOS]	TROUT ONCORHYNCHUS-MYKISS ; ANIMAL LECTINS ; SEQUENCE ALIGNMENT ; SKIN MUCUS ; EGGS ; FAMILY ; SUPERFAMILY ; EXPRESSION ; GALECTINS ; ACID
收录类别	SCI
资助信息	National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022]
语种	英语
WOS记录号	WOS:000281087300016
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13595]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Guangdong Pharmaceut Univ, Sch Life Sci, Guangzhou 510006, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Jia, W. Z.,Shang, N.,Guo, Q. L.. Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2010,36(3):451-459.
APA	Jia, W. Z.,Shang, N.,&Guo, Q. L..(2010).Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus.FISH PHYSIOLOGY AND BIOCHEMISTRY,36(3),451-459.
MLA	Jia, W. Z.,et al."Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus".FISH PHYSIOLOGY AND BIOCHEMISTRY 36.3(2010):451-459.